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-	Vector files are in ApE format saved as .gb files. The VF2 to Prefix has been moved to the beginning to facilitate sequencing alignments.	+	Vector files are in ApE format saved as .gb files. The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.
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Revision as of 04:37, 25 September 2013

iGEM Vector Information

Vector	Copy Number	Equivalent	Antibiotic	Length	Genbank / ApE files
pSB1C3	100-300	ColE1,pMB1(pUC19)	Chloramphenicol	2070 bp	pSB1C3(std).gb
pSB1A3	100-300	ColE1,pMB1(pUC19)	Ampicillin	2155 bp	pSB1A3(std).gb
pSB3K3	20-30	p15A(pMR101)	Kanamycin	2750 bp	pSB3K3(std).gb
pSB4A5	~5	RepA(pSC101)	Ampicillin	2037 bp	pSB4A5(std).gb
pSB3T5	20-30	p15A(pMR101)	Tetracycline	3252 bp	pSB3T5(std).gb

Vector files are in ApE format saved as .gb files. The VF2 to Prefix region has been moved to the beginning of the file to facilitate sequencing alignments.

Vector Equivalency Chart

Source Vector	Origin	Copy Number	Antibiotic	Biobrick Equivalent	Antibiotic
pJT119b	ColE1,pMB1	100-300	Chloramphenicol	pSB1C3	Chloramphenicol
pPLPCB	p15A	20-30	Spectinomycin	pSB3T5	Tetracycline
pEO100c	pSC101	~5	Ampicillin	pSB4A5	Ampicillin
pJT106b	pSC101	~5	Ampicillin	pSB4A5	Ampicillin