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Template:Kyoto/Notebook/Sep 3
From 2013.igem.org
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|9/2 14:00 pT181 attenuator||418.4||1.82||1.80 | |9/2 14:00 pT181 attenuator||418.4||1.82||1.80 | ||
|- | |- | ||
| - | |9/2 14:00 pT181 antisense||362.2||1. | + | |9/2 14:00 pT181 antisense||362.2||1.92||1.96 |
| + | |- | ||
| + | |9/2 14:00 Spinach||438.3||1.86||1.99 | ||
| + | |- | ||
| + | |9/2 14:00 tetR-apt 12_1R||567.5||1.90||1.99 | ||
| + | |- | ||
| + | |9/2 21:00 pT181 attenuater||320.9||1.90||2.15 | ||
| + | |- | ||
| + | |9/2 21:00 Spinach||457.0||1.88||2.24 | ||
|} | |} | ||
</div> | </div> | ||
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===Liquid Culture=== | ===Liquid Culture=== | ||
===Restriction Enzyme Digestion=== | ===Restriction Enzyme Digestion=== | ||
Revision as of 09:26, 25 September 2013
Contents |
Sep 3
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | pT181 attenuater(2)-DT2(738) |
| 3 | Ptet-RBS-lacZα-DT-2(756) |
| 4 | Ptet-RBS-lacZα-DT-3 |
| 5 | Ptet-RBS-lacZα-DT-4 |
| 6 | Ptet-RBS-lacZα-DT-5 |
| 7 | Ptet-RBS-lacZα-DT-6 |
| 8 | RBS-lysis2-DT-2(985) |
| 9 | RBS-lysis2-DT-3 |
| 10 | 100bp ladder |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 9/2 14:00 pT181 attenuator | 418.4 | 1.82 | 1.80 |
| 9/2 14:00 pT181 antisense | 362.2 | 1.92 | 1.96 |
| 9/2 14:00 Spinach | 438.3 | 1.86 | 1.99 |
| 9/2 14:00 tetR-apt 12_1R | 567.5 | 1.90 | 1.99 |
| 9/2 21:00 pT181 attenuater | 320.9 | 1.90 | 2.15 |
| 9/2 21:00 Spinach | 457.0 | 1.88 | 2.24 |
