Team:Buenos Aires/ model

From 2013.igem.org

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(Deterministic Model)
(Introduction)
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More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water.
More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water.
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We worked with Arsenic knowing that an arsenic detoxification operon (Ars operon) exist in nature and more important, it exists as biobricks.
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We worked with Arsenic knowing that an arsenic detoxification operon (Ars operon) exist in nature and more important, it exists as a biobricks.
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=='''Got Ideas?'''==
=='''Got Ideas?'''==

Revision as of 13:14, 25 September 2013


Contents

Deterministic Model

Introduction

Our objective was to assemble a system that could respond to different concentrations of a specific contaminant in water. More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water.

We worked with Arsenic knowing that an arsenic detoxification operon (Ars operon) exist in nature and more important, it exists as a biobricks.

Got Ideas?

For our biosensor we came up with three different designs each one motivated on improving the flaws detected while analysing the previous ones using mathematical models as our main tools. We describe briefly the first two designs, which were rejected, to emphasize the importance of mathematical model to gain insight on how our system works and as a feasibility study taking into considerations .

Arsenic promoter + ArsR + Reporter

First we tried to keep it as simple as we could. Figure 1 shows our first design which, in theory, produces different responses to different concentrations of arsenic in water. Figure 2 shows a simulation graphing the time series response of the Pars promoter-arsR for different concentrations of ars.

This result is true but misleading because we are not seeing the whole picture. Althought we have a system that recognise and respond differently to different concentrations of arsenic since our reporter was meant to be a coloured pigment (the reasons for this are detailed in the project tab) scale tuning needs to be taken into account.

The reporter is downstream the Pars promoter and the design shows little flexibility, i.e. few available parameters, to produce a discernable coloured output.


Unfortunately

Rfp en el tiempo.jpg

As it is shown in the figure below, in the presence of arsenite (1000 ppb) a typical transcriptional induction is observed over time. There is a lag of 3 hours in the mRFP produciton after adding arsenite.

mRFP stability over time