Template:Kyoto/Notebook/Sep 21

From 2013.igem.org

(Difference between revisions)
(→Electrophoresis)
(→Liquid Culture)
Line 29: Line 29:
|9/5 Pcon-pT181 attenuator-1||--
|9/5 Pcon-pT181 attenuator-1||--
|}
|}
 +
</div>
 +
 +
===Master Plate===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{| class="wikitable"
 +
!Number||Use LB plate
 +
|-
 +
|1||8/27 P&lambda;-luxI-1(+Amp)
 +
|-
 +
|2||9/10 Plac(BBa-R0011)-3(+Amp)
 +
|-
 +
|3||9/5 Pcon-RBS-tetR-DT-1(+Amp)
 +
|-
 +
|4||9/5 Pcon-pT181 antisense-1(+Amp)
 +
|-
 +
|5||9/15 Pcon-pT181 attenuator(+Amp)
 +
|-
 +
|6||9/- Pcon-apt12_1R-DT-1(+Amp)
 +
|-
 +
|7||9/5 Pcon-spinach-DT-3(+Amp)
 +
|-
 +
|8||8/27 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(+Amp)
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Number||Use LB plate
 +
|-
 +
|1||9/4 pT181 antisense(pSB1C3)-1(+CP)
 +
|-
 +
|2||9/11 Fusion1 antisense(pSB1C3)-4(+CP)
 +
|-
 +
|3||9/11 Fusion6 antisense(pSB1C3)-1(+CP)
 +
|-
 +
|4||9/4 pT181 attenuator(pSB1C3)-1(+CP)
 +
|-
 +
|5||9/10 Fusion1 attenuator(pSB1C3)-1(+CP)
 +
|-
 +
|6||9/11 Fusion3n2 attenuator(pSB1C3)-1(+CP)
 +
|-
 +
|7||9/4 aptamer12_1R(pSB1C3)-1(+CP)
 +
|-
 +
|8||9/11 aptamer12_P(pSB1C3)-3(+CP)
 +
|-
 +
|9||9/11 aptamer12_1M(pSB1C3)-1(+CP)
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Number||Use LB plate
 +
|-
 +
|1||8/29 Pbad/araC-RBS-RFP-DT-1(+CP)
 +
|-
 +
|2||9/4 RBS-lysis3-DT-3(+CP)
 +
|-
 +
|3||9/10 Pcon-pT181 attenuator-DT-1(+CP)
 +
|-
 +
|4||9/5 Ptet-pT181 antisense-1(+CP)
 +
|-
 +
|5||9/4aptamer 12_1R-DT-1(+CP)
 +
|-
 +
|6||9/5 Ptet-RBS-lacZ&alpha;-DT-4(+CP)
 +
|-
 +
|7||8/10 RBS-lacI-DT-2(+CP)
 +
|-
 +
|8||8/29 Plux-RBS-GFP-DT-1(+CP)
 +
|}
</div>
</div>

Revision as of 14:50, 25 September 2013

Contents

Sep 21

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
1Pcon-RBS-lacZα-DT----
21kbp ladder----
3pSB1C3+pT181 attenuator----

File:Igku xxxxxx.xxx

Liquid Culture

No name

Samplemedium
9/2 spinach-DT-2--
8/16 Plux-4--
8/27 Plux-RBS-GFP-DT-1--
9/5 Pcon-pT181 attenuator-1--

Master Plate

No name

NumberUse LB plate
18/27 Pλ-luxI-1(+Amp)
29/10 Plac(BBa-R0011)-3(+Amp)
39/5 Pcon-RBS-tetR-DT-1(+Amp)
49/5 Pcon-pT181 antisense-1(+Amp)
59/15 Pcon-pT181 attenuator(+Amp)
69/- Pcon-apt12_1R-DT-1(+Amp)
79/5 Pcon-spinach-DT-3(+Amp)
88/27 Pcon-RBS-GFP-DT-Pcon-RBS-luxR-DT(+Amp)
NumberUse LB plate
19/4 pT181 antisense(pSB1C3)-1(+CP)
29/11 Fusion1 antisense(pSB1C3)-4(+CP)
39/11 Fusion6 antisense(pSB1C3)-1(+CP)
49/4 pT181 attenuator(pSB1C3)-1(+CP)
59/10 Fusion1 attenuator(pSB1C3)-1(+CP)
69/11 Fusion3n2 attenuator(pSB1C3)-1(+CP)
79/4 aptamer12_1R(pSB1C3)-1(+CP)
89/11 aptamer12_P(pSB1C3)-3(+CP)
99/11 aptamer12_1M(pSB1C3)-1(+CP)

</div>

NumberUse LB plate
18/29 Pbad/araC-RBS-RFP-DT-1(+CP)
29/4 RBS-lysis3-DT-3(+CP)
39/10 Pcon-pT181 attenuator-DT-1(+CP)
49/5 Ptet-pT181 antisense-1(+CP)
59/4aptamer 12_1R-DT-1(+CP)
69/5 Ptet-RBS-lacZα-DT-4(+CP)
78/10 RBS-lacI-DT-2(+CP)
88/29 Plux-RBS-GFP-DT-1(+CP)

</div>

Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_21"