Team:AITM-Nepal/Part3

From 2013.igem.org

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#logo
#logo
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ul#navigation
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.navbar
{
{
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{
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ul.sub_navigation li
ul.sub_navigation li
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/* navbar css complete */
 
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#box
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/* navbar css complete */
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</style>
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<script>
<script>
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</head>
</head>
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<div class="AllContent">
<!-- Logo Section Starts -->
<!-- Logo Section Starts -->
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<img id="logo" src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png">
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<div class="header">             
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    <img id="logo" src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png">
<img src="https://static.igem.org/mediawiki/2013/8/8e/1186269_700314813316746_1614415077_n.png" width="500px" height="140px" style="margi:auto;">
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<a href="https://2013.igem.org/Main_Page"><img id="logo2" src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png"></a>
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    <a href="https://2013.igem.org/Main_Page"><img id="logo2" src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png"></a>
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<!-- Logo Section Complete -->
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<!-- Navbar Section Start -->
<!-- Navbar Section Start -->
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<div class="navbar">
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    <ul id="navigation">
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    <div id="navigationboxholder">
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        <ul id="navigation">
                  
                  
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                 <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal"><font color="#000000" face="Georgia">Home</font></a></li>
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                 <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal">Home</a></li>
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                 <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Team"><font color="#000000" face="Georgia">Team</font></a></li>
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                 <li><a  class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Team">Team</a></li>
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                 <li><a onMouseOver="togglemenu();" href="https://2013.igem.org/Team:AITM-Nepal/Project" class="navbar-option">Project</a>
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                <li><a class="dropdown" onMouseOver="togglemenu();" href="https://2013.igem.org/Team:AITM-Nepal/Project"><font color="#000000" face="Georgia">Project</font></a>
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                             <ul class="sub_navigation">
                             <ul class="sub_navigation">
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part1">Part 1</a></li>
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part1" class="navbar-option">Part 1</a></li>
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part2">Part 2</a></li>
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part2" class="navbar-option">Part 2</a></li>
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part3">Part 3</a></li>
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                                     <li><a href="https://2013.igem.org/Team:AITM-Nepal/Part3" class="navbar-option">Part 3</a></li>
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                              </ul></li>
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                            </ul></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Parts"><font color="#000000" face="Georgia">Parts Submitted To Registry</font></a></li>
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                 <li><a class="navbar-option" href="https://igem.org/Team.cgi?year=2013&team_name=AITM-Nepal">Official Team Profile</a></li>
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                <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Parts">Parts Submitted To Registry</a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Notebook"><font color="#000000" face="Georgia">Notebook</font></a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Modeling">Modeling</a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Safety"><font color="#000000" face="Georgia">Safety</font></a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Human_practise"><font color="#000000" face="Georgia">Human Practice</font></a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Attributions"><font color="#000000" face="Georgia">Attributions</font></a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Human_practise">Human Practice</a></li>
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                 <li><a class="navbar-option" href="https://2013.igem.org/Team:AITM-Nepal/Attributions">Attributions</a></li>
     </ul>
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<h2> Part 3</h2>
<h2> Part 3</h2>
<p>Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease3. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. When expressed in E. coli, p19 stabilizes an ~21-nt siRNA-like species produced by bacterial RNase III. When mammalian cells are transfected by them, siRNAs that were generated in bacteria expressing p19 and a hairpin RNA encoding 200 or more nucleotides of a target gene reproducibly knock down target gene expression by ~90% . Because bacterially produced siRNAs contain multiple sequences against a target gene, they may be especially useful for suppressing polymorphic cellular or viral genes.</p>
<p>Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease3. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. When expressed in E. coli, p19 stabilizes an ~21-nt siRNA-like species produced by bacterial RNase III. When mammalian cells are transfected by them, siRNAs that were generated in bacteria expressing p19 and a hairpin RNA encoding 200 or more nucleotides of a target gene reproducibly knock down target gene expression by ~90% . Because bacterially produced siRNAs contain multiple sequences against a target gene, they may be especially useful for suppressing polymorphic cellular or viral genes.</p>
</div>
</div>

Revision as of 17:16, 25 September 2013

Part 3

Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease3. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. When expressed in E. coli, p19 stabilizes an ~21-nt siRNA-like species produced by bacterial RNase III. When mammalian cells are transfected by them, siRNAs that were generated in bacteria expressing p19 and a hairpin RNA encoding 200 or more nucleotides of a target gene reproducibly knock down target gene expression by ~90% . Because bacterially produced siRNAs contain multiple sequences against a target gene, they may be especially useful for suppressing polymorphic cellular or viral genes.

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