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Team:Shenzhen BGIC ATCG/results
From 2013.igem.org
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<p> Using the GFP as reporter and morphological alteration as cell cycle representation, we verified the Clb6 can be activated in G1 phase in the yeast.</P> | <p> Using the GFP as reporter and morphological alteration as cell cycle representation, we verified the Clb6 can be activated in G1 phase in the yeast.</P> | ||
<P> As shown in the picture in normal lights, there are some yeast were budding. These budded yeast cells and small size budding yeasts (within the red circles) are assumed in the G1 phase of cell cycle. And the next picture was taken under activation light, thus the green lights can representing the Clb6 promoter expressing. The two pictures indicated the Clb6 were expressed in the G1 phase as we expected when designing the experiments </p> | <P> As shown in the picture in normal lights, there are some yeast were budding. These budded yeast cells and small size budding yeasts (within the red circles) are assumed in the G1 phase of cell cycle. And the next picture was taken under activation light, thus the green lights can representing the Clb6 promoter expressing. The two pictures indicated the Clb6 were expressed in the G1 phase as we expected when designing the experiments </p> | ||
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| + | <img scr="https://static.igem.org/mediawiki/2013/4/41/Clb6.jpg" /> | ||
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| + | <p>预留流式细胞仪图</p> | ||
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<div id="box3" class="box"> | <div id="box3" class="box"> | ||
<h3>Reporter Locating</h3> | <h3>Reporter Locating</h3> | ||
| + | <p>预留荧光蛋白图</p> | ||
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| + | <img scr="https://static.igem.org/mediawiki/2013/6/6e/Target2.jpg" /> | ||
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<div id="box4" class="box"> | <div id="box4" class="box"> | ||
<h3>Degradation Rate</h3><br/> | <h3>Degradation Rate</h3><br/> | ||
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| + | <img src="https://static.igem.org/mediawiki/2013/b/b0/Curve_ecdeg.png" /> | ||
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| + | <img src="https://static.igem.org/mediawiki/2013/a/a6/IMG_0804.jpg" /> | ||
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| + | <img src="https://static.igem.org/mediawiki/2013/c/ca/IMG_0817.jpg" /> | ||
</div> | </div> | ||
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<div id="box5" class="box"> | <div id="box5" class="box"> | ||
<h3>Cell Synchronization</h3> | <h3>Cell Synchronization</h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/e/e4/09172_bright.jpg" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/3/3f/Sc_bright_20.jpg" /> | ||
</div> | </div> | ||
Revision as of 20:03, 25 September 2013
Playing with my eyes
aren't you?
Hi I am Dr. Mage!
A "budding" yeast cell!
The Magic
Our project “Cell Magic", a complex work has been completed partly,due to the time limitation. The results we have gotten are listed below:
We verified the Clb6 promoter, which is supposed to be expressed in G1 phase.
Promoter Verification
Using the GFP as reporter and morphological alteration as cell cycle representation, we verified the Clb6 can be activated in G1 phase in the yeast.
As shown in the picture in normal lights, there are some yeast were budding. These budded yeast cells and small size budding yeasts (within the red circles) are assumed in the G1 phase of cell cycle. And the next picture was taken under activation light, thus the green lights can representing the Clb6 promoter expressing. The two pictures indicated the Clb6 were expressed in the G1 phase as we expected when designing the experiments
预留流式细胞仪图
Reporter Locating
预留荧光蛋白图
Degradation Rate
Cell Synchronization
