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Team:NJU China/Notebook
From 2013.igem.org
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<a>siRNA screening (Failed)</a> | <a>siRNA screening (Failed)</a> | ||
<span>14th: We extracted plasmids and cultured the 293t cells.</span> | <span>14th: We extracted plasmids and cultured the 293t cells.</span> | ||
<span>15th: We transfected plasmids into 293t cells.</span> | <span>15th: We transfected plasmids into 293t cells.</span> | ||
| - | <span>We | + | <span>16th: We collected cells and preserved it in Trizol.</span> |
| - | <span>We | + | <span>17th: We extracted RNA, then did RT-PCR with the RNA.</span> |
| - | <span>We | + | <span>18th: We did qPCR with the cDNA we got on 17th April.</span> |
| + | <span>21th: We reexamined the concentration of RNA, and redid qPCR.</span> | ||
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Revision as of 23:06, 25 September 2013
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Notebook
March
2013
Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.
October
2013
siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.
October 24, 2013 Creative CSS3 Animation Menus
October 19, 2013 Blur Menu with CSS3 Transitions
October 17, 2013 Wave Display Effect with jQuery
October 12, 2013 Flexible Slide-to-top Accordion
October 10, 2013 Circle Navigation Effect with CSS3
October 7, 2013 Draggable Image Boxes Grid
September
2013
September 30, 2013 Scrollbar Visibility with jScrollPane
September 27, 2013 Multi-level Photo Map
September 20, 2013 Responsive Image Gallery with Thumbnail Carousel
September 12, 2013 Elastislide - A Responsive jQuery Carousel Plugin
September 5, 2013 Slicebox - A fresh 3D image slider with graceful fallback
August
2013
August 23, 2013 Image Zoom Tour with jQuery
July
2013
July 29, 2013 Content Rotator with jQuery
July 5, 2013 Fullscreen Slideshow with HTML5 Audio and jQuery
June
2013
来源：站长素材
