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Team:USTC CHINA/Notebook/Protocols/Gel Extraction
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Revision as of 00:29, 26 September 2013
Gel Extraction
Performed with AxyPrep 96-well DNA Gel Extraction Kit (type: AP-96-GX)
Protocol
1. Excise gel slice containing DNA fragment of interest.
2. Add 3×sample volume of Buffer DE-A.
Incubate at 75° C for 15-20 min or until gel melts completely.
Add 0.5 × Buffer DE-A volume of Buffer DE-B.
3. Binding sample DNA(Centrifuge at 5,000 - 6,000×g for 5 min).
4. Add 800 μl of Buffer W2 (5,000 - 6,000×g for 1 min).
Repeat wash with Buffer W2.
Centrifuge empty plate for 5 min at 5,000 - 6,000×g to remove residue W2.
5. Elute with 50 μl of Eluent or Deionized Water.(5,000 - 6,000×g, 5min).
