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Team:UChicago/Protocols
From 2013.igem.org
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==General Lab Best Practices== | ==General Lab Best Practices== | ||
| + | |||
| + | ===Labeling=== | ||
| + | |||
| + | Label tubes at all time before storage. Label clearly with: | ||
| + | |||
| + | #Contents in the tube | ||
| + | #Date | ||
| + | #Your name/Initials | ||
| + | #Number | ||
| + | |||
| + | |||
| + | ===Notes on Sterile Techniques=== | ||
| + | |||
| + | *Turn the flame on before opening anything sterile. | ||
| + | |||
| + | *Aliquot everything you use to prevent contamination. Before pouring aliquots, flame the bottle rim quickly to keep sterile. Make new aliquots in new tubes with stock when running low. | ||
| + | |||
| + | *Unscrew but do not remove all caps to make it easier to work with in the moment. | ||
| + | |||
| + | *Flame the bottle rim quickly and then aliquot by pouring into 50ml conical tube. Never stick anything in LB bottle, always pour. | ||
| + | |||
| + | *Flame bottle rim again and close LB bottle. | ||
| + | |||
| + | *Take what you need from 50ml tube, keep sterile so it can be used repeatedly. | ||
==Lab Notebook Best Practices (read: do these best practices)== | ==Lab Notebook Best Practices (read: do these best practices)== | ||
Revision as of 03:42, 26 September 2013
Notebook > Protocols
Protocols page
General Lab Best Practices
Labeling
Label tubes at all time before storage. Label clearly with:
- Contents in the tube
- Date
- Your name/Initials
- Number
Notes on Sterile Techniques
- Turn the flame on before opening anything sterile.
- Aliquot everything you use to prevent contamination. Before pouring aliquots, flame the bottle rim quickly to keep sterile. Make new aliquots in new tubes with stock when running low.
- Unscrew but do not remove all caps to make it easier to work with in the moment.
- Flame the bottle rim quickly and then aliquot by pouring into 50ml conical tube. Never stick anything in LB bottle, always pour.
- Flame bottle rim again and close LB bottle.
- Take what you need from 50ml tube, keep sterile so it can be used repeatedly.
Lab Notebook Best Practices (read: do these best practices)
Steps to 3A Assembly & Labeling Guidelines
Resuspending DNA from iGEM Kit Plates
Recipes
Making Agar Plates
Pouring the Plates
Making LB (500mL)
Agar Stab Protocols
Ligation Protocol
Transformation Procedures
Making Overnight Cultures
Miniprep Protocols
Making an agarose gel for gel purification
Enzymatic digestion
Gel extraction with the QIAGEN kit
