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Team:UChicago/Protocols
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#'''Conclusion''': was your experiment successful? why not? what’s next? what did you expect to see? | #'''Conclusion''': was your experiment successful? why not? what’s next? what did you expect to see? | ||
#'''Your name''': so others can ask questions to the right person. This is one reason why it’s critical to label tubes with the date, description, and your name | #'''Your name''': so others can ask questions to the right person. This is one reason why it’s critical to label tubes with the date, description, and your name | ||
| + | |||
'''Best practices from Thomson’s [http://www.iphandbook.org/handbook/chPDFs/ch08/ipHandbook-Ch%2008%2002%20Thomson%20Laboratory%20Notebooks.pdf How to Start–and Keep–a Laboratory Notebook: Policy and Practical Guidelines]''' (see link and scroll down for examples): | '''Best practices from Thomson’s [http://www.iphandbook.org/handbook/chPDFs/ch08/ipHandbook-Ch%2008%2002%20Thomson%20Laboratory%20Notebooks.pdf How to Start–and Keep–a Laboratory Notebook: Policy and Practical Guidelines]''' (see link and scroll down for examples): | ||
| - | “Although you may think you will remember what you did and why you did a certain experiment in a week’s time, YOU WILL NOT! And nor will anyone else in your laboratory. Hence the need for laboratory notebooks. In short, a laboratory notebooks is: | + | <blockquote>“Although you may think you will remember what you did and why you did a certain experiment in a week’s time, YOU WILL NOT! And nor will anyone else in your laboratory. Hence the need for laboratory notebooks. In short, a laboratory notebooks is: |
| - | a daily record of every experiment you do, think of doing, or plan to do | + | *a daily record of every experiment you do, think of doing, or plan to do |
| - | a daily record of your thoughts about each experiment and the results thereof | + | *a daily record of your thoughts about each experiment and the results thereof |
| - | a record that would enable successive scientists, working on the same project, to pick up where you left off or reproduce your results” | + | *a record that would enable successive scientists, working on the same project, to pick up where you left off or reproduce your results”</blockquote> |
| - | “What goes into a laboratory notebook? | + | <blockquote>'''“What goes into a laboratory notebook?''' |
| - | a detailed account of every planned and executed experiment with the amount of detail that would enable a skilled scientist to determine what had been done, why it had been done, and what the results were | + | *a detailed account of every planned and executed experiment with the amount of detail that would enable a skilled scientist to determine what had been done, why it had been done, and what the results were |
| - | dates accompanying every entry, account, or record | + | *dates accompanying every entry, account, or record |
| - | [Links to outside resources used, formulas used for any calculations. -Alice] | + | *[Links to outside resources used, formulas used for any calculations. -Alice] |
| - | explanations of the significance of each experiment, as well as the observations, results and conclusions of the experiment | + | *explanations of the significance of each experiment, as well as the observations, results and conclusions of the experiment |
| - | details of each experiment (Remember, what may seem trivial or obvious at the time your experiment was conducted, may later be of critical importance.) | + | *details of each experiment (Remember, what may seem trivial or obvious at the time your experiment was conducted, may later be of critical importance.) |
| - | personal comments (It is a living document, so stamp it with your own personality. Comments such as “SUCCESS AT LAST!! THIRD TIME LUCKY :)” are highly appropriate. | + | *personal comments (It is a living document, so stamp it with your own personality. Comments such as “SUCCESS AT LAST!! THIRD TIME LUCKY :)” are highly appropriate. |
| - | photographs, computer generated data, and so forth should all be stuck into your notebook. a good photo matters! | + | *photographs, computer generated data, and so forth should all be stuck into your notebook. a good photo matters! |
| - | cross-references [For example, if you are starting a new experiment on 8/21 entry and are using the same protocol as already described in the Binder of Lab Practices, write on 8/21 entry, “following the protocol as described in the Binder of Lab Practices” -Alice] | + | *cross-references [For example, if you are starting a new experiment on 8/21 entry and are using the same protocol as already described in the Binder of Lab Practices, write on 8/21 entry, “following the protocol as described in the Binder of Lab Practices” -Alice]"</blockquote> |
| - | “A laboratory notebook is an important tool that goes well beyond research management, and keeping good records has implications for issues ranging from intellectual property management to the prevention of fraud.” | + | <blockquote>“A laboratory notebook is an important tool that goes well beyond research management, and keeping good records has implications for issues ranging from intellectual property management to the prevention of fraud.”</blockquote> |
==Steps to 3A Assembly & Labeling Guidelines== | ==Steps to 3A Assembly & Labeling Guidelines== | ||
Revision as of 04:02, 26 September 2013
Notebook > Protocols
Protocols page
General Lab Best Practices
Labeling
Label tubes at all time before storage. Label clearly with:
- Contents in the tube
- Date
- Your name/Initials
- Number
Notes on Sterile Techniques
- Turn the flame on before opening anything sterile.
- Aliquot everything you use to prevent contamination. Before pouring aliquots, flame the bottle rim quickly to keep sterile. Make new aliquots in new tubes with stock when running low.
- Unscrew but do not remove all caps to make it easier to work with in the moment.
- Flame the bottle rim quickly and then aliquot by pouring into 50ml conical tube. Never stick anything in LB bottle, always pour.
- Flame bottle rim again and close LB bottle.
- Take what you need from 50ml tube, keep sterile so it can be used repeatedly.
Lab Notebook Best Practices
Every lab notebook entry should contain…
- Title: what technique you’re using, what you’re using it on (refer to specific tubes*)
- Reference to protocol: are you using the standard protocol found in the masterlist?
- Changes in protocol: have you done anything different from the standard protocol?
- Conditions: did you run at 120V? for how long?
- Mistakes: in case an experiment doesn’t work as a result of a mistake
- Conclusion: was your experiment successful? why not? what’s next? what did you expect to see?
- Your name: so others can ask questions to the right person. This is one reason why it’s critical to label tubes with the date, description, and your name
Best practices from Thomson’s [http://www.iphandbook.org/handbook/chPDFs/ch08/ipHandbook-Ch%2008%2002%20Thomson%20Laboratory%20Notebooks.pdf How to Start–and Keep–a Laboratory Notebook: Policy and Practical Guidelines] (see link and scroll down for examples):
“Although you may think you will remember what you did and why you did a certain experiment in a week’s time, YOU WILL NOT! And nor will anyone else in your laboratory. Hence the need for laboratory notebooks. In short, a laboratory notebooks is:
- a daily record of every experiment you do, think of doing, or plan to do
- a daily record of your thoughts about each experiment and the results thereof
- a record that would enable successive scientists, working on the same project, to pick up where you left off or reproduce your results”
“What goes into a laboratory notebook?
- a detailed account of every planned and executed experiment with the amount of detail that would enable a skilled scientist to determine what had been done, why it had been done, and what the results were
- dates accompanying every entry, account, or record
- [Links to outside resources used, formulas used for any calculations. -Alice]
- explanations of the significance of each experiment, as well as the observations, results and conclusions of the experiment
- details of each experiment (Remember, what may seem trivial or obvious at the time your experiment was conducted, may later be of critical importance.)
- personal comments (It is a living document, so stamp it with your own personality. Comments such as “SUCCESS AT LAST!! THIRD TIME LUCKY :)” are highly appropriate.
- photographs, computer generated data, and so forth should all be stuck into your notebook. a good photo matters!
- cross-references [For example, if you are starting a new experiment on 8/21 entry and are using the same protocol as already described in the Binder of Lab Practices, write on 8/21 entry, “following the protocol as described in the Binder of Lab Practices” -Alice]"
“A laboratory notebook is an important tool that goes well beyond research management, and keeping good records has implications for issues ranging from intellectual property management to the prevention of fraud.”
Steps to 3A Assembly & Labeling Guidelines
Resuspending DNA from iGEM Kit Plates
Recipes
Making Agar Plates
Pouring the Plates
Making LB (500mL)
Agar Stab Protocols
Ligation Protocol
Transformation Procedures
Making Overnight Cultures
Miniprep Protocols
Making an agarose gel for gel purification
Enzymatic digestion
Gel extraction with the QIAGEN kit
