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Team:NTNU-Trondheim/Protocols
From 2013.igem.org
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== Protocols == | == Protocols == | ||
| + | ====Transformation==== | ||
| + | |||
| + | =====Materials===== | ||
| + | *Resuspended DNA (''Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes'') | ||
| + | *[[Help:Protocols/Competent_Cells | Competent cells]] (''50ul per transformation'') | ||
| + | *Ice (in ice bucket/container) | ||
| + | *2ml tube (''1 per a transformation''') | ||
| + | *42ºC water bath | ||
| + | *SOC media (''check for contamination!'') | ||
| + | *Petri dishes with LB agar and appropriate antibiotic (''2 per transformation'') | ||
| + | *glass beads or spreader | ||
| + | *37ºC incubator | ||
| + | *10pg/ul RFP Control (pSB1A3 w/ BBa_J04450) | ||
== more protocols == | == more protocols == | ||
Revision as of 08:49, 21 June 2013
