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Team:NTNU-Trondheim/Protocols
From 2013.igem.org
(Difference between revisions)
(→Laboratory Protocols) |
(→Laboratory Protocols) |
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We used this protocol with these modifications: | We used this protocol with these modifications: | ||
*Heat-shock 45 seconds instead of 60 seconds | *Heat-shock 45 seconds instead of 60 seconds | ||
| - | *Incubation for min 1 hour in shaker at 37°, not 2 hours. | + | *Incubation for min 1 hour in shaker at 37°C, not 2 hours. |
*LB medium instead of SOC. | *LB medium instead of SOC. | ||
Revision as of 09:03, 21 June 2013
