Friday, 05 Jul 2013

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=='''7/05/13 Primer Design for kerA biobrick'''==
 
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*Designed primers for directed mutagenesis of our kerA biobrick using [http://www.bioinformatics.org/primerx/ PrimerX]:
 
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#Primer pair 1 (EcoRI mutation, aat --> aac, asparagine)
 
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#:a. Forward: 5' GGTTAAAGTACTGAACTCAAGCGGAAGCGG 3'
 
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#:b. Reverse: 5' CCGCTTCCGCTTGAGTTCAGTACTTTAACC 3'
 
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#Primer pair 2 (Pst1 mutation, gca-->gcg, alanine)
 
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#:a. Forward: 5' GTTGTCGTTGTAGCTGCGGCAGGGAACAGCGGATC 3'
 
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#:b. Reverse: 5' GATCCGCTGTTCCCTGCCGCAGCTACAACGACAAC 3'
 
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*Used APE for checking codons and mutating restriction sites
 

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