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Template:Kyoto/Notebook/Sep 26
From 2013.igem.org
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!state||colspan="2"|Vector||colspan="2"|Inserter1||colspan="2"|Inserter2||colspan="2"|Inserter3||colspan="2"|Inserter4||Ligation High ver.2 | !state||colspan="2"|Vector||colspan="2"|Inserter1||colspan="2"|Inserter2||colspan="2"|Inserter3||colspan="2"|Inserter4||Ligation High ver.2 | ||
|- | |- | ||
| - | |experiment||RBS-GFP-DT||1.0 µL||Pcon-pT 181 antisense(E-2A)||0.6µL||DT(2-1)||0.5µL||Pcon-pT181 attenuator(1-SA)||2.1 µL||2.1 µL | + | |experiment||RBS-GFP-DT||1.0 µL||Pcon-pT 181 antisense(E-2A)||0.6µL||DT(2-1)||0.5µL||Pcon-pT181 attenuator(1-SA)||2.1 µL||--||2.1 µL |
|- | |- | ||
| - | |experiment||pSB1C3(EcoRI&SpeI)||1.8 µL||Pcon-pT 181 antisense(E-2A)||0.9 µL||DT(2-1)||0.5 µL||Pcon-GFP-DT(1-SA)||1.5 µL||2.5 µL | + | |experiment||pSB1C3(EcoRI&SpeI)||1.8 µL||Pcon-pT 181 antisense(E-2A)||0.9 µL||DT(2-1)||0.5 µL||Pcon-GFP-DT(1-SA)||1.5 µL||--||2.5 µL |
|- | |- | ||
|experiment||9/13 Pcon-RBS-luxR-DT||2.1 µL||9/10 Plux-RBS-GFP-DT||5.6 µL||3.5 µL | |experiment||9/13 Pcon-RBS-luxR-DT||2.1 µL||9/10 Plux-RBS-GFP-DT||5.6 µL||3.5 µL | ||
Revision as of 08:37, 26 September 2013
Sep 26
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 | |
|---|---|---|---|---|
| 1 | GGA1-1 | |||
| 2 | GGA1-2 | |||
| 3 | GGA1-3 | |||
| 4 | GGA1-4 | |||
| 5 | GGA1-5 | |||
| 6 | GGA1-6 | |||
| 7 | GGA1-7 | |||
| 8 | GGA1-8 | |||
| 9 | 1kbp ladder | |||
| 10 | GGA2-1 | |||
| 11 | GGA2-2 | |||
| 12 | GGA2-3 | |||
| 13 | GGA2-4 | |||
| 14 | GGA2-5 | |||
| 15 | GGA2-6 | |||
| 16 | GGA2-7 | |||
| 17 | GGA2-8 |
| Lane | Sample | Enzyme1 | Enzyme2 | |
|---|---|---|---|---|
| 1 | GGA3-1 | |||
| 2 | GGA3-2 | |||
| 3 | GGA3-3 | |||
| 4 | 1kbp ladder | |||
| 5 | GGA3-4 | |||
| 6 | GGA3-5 | |||
| 7 | GGA3-6 | |||
| 8 | GGA3-7 |
File:Xxxxx.png
</div>
| Lane | Sample | Enzyme1 | Enzyme2 | |
|---|---|---|---|---|
| 1 | GGA3-8 | |||
| 2 | GGA4-1 | |||
| 3 | GGA4-2 | |||
| 4 | GGA4-3 | |||
| 5 | 1kbp ladder | |||
| 6 | GGA4-4 | |||
| 7 | GGA4-5 | |||
| 8 | GGA4-6 |
File:Xxxxx.png
</div>
| Lane | Sample | Enzyme1 | Enzyme2 | |
|---|---|---|---|---|
| 1 | GGA4-7 | |||
| 2 | GGA4-8 | |||
| 3 | 1kbp ladder | |||
| 4 | GGA7-1 | |||
| 5 | GGA16-1 | |||
| 6 | NC(RBS-GFP-DT) |
File:Xxxxx.png
</div>
Ligasion(Golden Gate Assenbly)
| state | Vector | Inserter1 | Inserter2 | Inserter3 | Inserter4 | Ligation High ver.2 | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| experiment | RBS-GFP-DT | 1.0 µL | Pcon-pT 181 antisense(E-2A) | 0.6µL | DT(2-1) | 0.5µL | Pcon-pT181 attenuator(1-SA) | 2.1 µL | -- | 2.1 µL | |
| experiment | pSB1C3(EcoRI&SpeI) | 1.8 µL | Pcon-pT 181 antisense(E-2A) | 0.9 µL | DT(2-1) | 0.5 µL | Pcon-GFP-DT(1-SA) | 1.5 µL | -- | 2.5 µL | |
| experiment | 9/13 Pcon-RBS-luxR-DT | 2.1 µL | 9/10 Plux-RBS-GFP-DT | 5.6 µL | 3.5 µL | ||||||
| experiment | 9/13 Pcon-pT181 attenuator (SpeI & PstI) | 2.0 µL | 9/18 aptamer 12-1R-DT (XbaI & PstI) | 6.3 µL | 3.5 µL | ||||||
| experiment | 9/18 Plac ②(SpeI & PstI) | 3.0 µL | 9/18 aptamer 12-1R-DT(XbaI & PstI) | 4.5µL | x3.5 µL | ||||||
