"
Team:Freiburg/Project/modeling
From 2013.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{:Template:Freiburg2013_Template1}} | {{:Template:Freiburg2013_Template1}} | ||
| - | + | ||
<html> | <html> | ||
<head> | <head> | ||
| Line 11: | Line 11: | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
| - | function CrispR (form) { | + | jQuery(document).ready(function CrispR (form){ |
| + | |||
var TestVar = form.Sequenz.value; | var TestVar = form.Sequenz.value; | ||
var length = TestVar.length; | var length = TestVar.length; | ||
| Line 170: | Line 171: | ||
} | } | ||
| + | }); | ||
</SCRIPT> | </SCRIPT> | ||
<style type="text/css"> | <style type="text/css"> | ||
Revision as of 09:13, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequenz for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.
