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{

ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1> Name of participants: Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer <h2>Ongoing constructs:</h2> 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-CP44 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA L. reuteri Lb4. 100-23 no plasmid Lb12. DSM 20016 no plasmid L. plantarum: Lb10. 256 no plasmid Lb11. 36E no plasmid L. thermophilus: Lb19. L.thermophilus Todays work Screening PCR & gel analysis of constructs: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 Plasmid prep & glycerol stock: 48. pSB1C3-B0034-His-STS O/N: 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 47.(9, 20) pSB1C3-B0034-STS Lb4. reuteri 100-23 no plasmid Lb10. plantarum 256 no plasmid Lb11. plantarum 36E no plasmid Lb12. reuteri DSM 20016 no plasmid Re-streaks: Lb19. L.thermophilus <h2>Results</h2> The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB. PCR screen and gel electrophoresis: 61.1 pSB1C3-CP1 61.2 pSB1C3-CP1 62.1 pSB1C3-CP8 62.2 pSB1C3-CP8 62.3 pSB1C3-CP8 62.4 pSB1C3-CP8 63.1 pSB1C3-CP11 63.2 pSB1C3-CP11 63.3 pSB1C3-CP11 63.4 pSB1C3-CP11 64.1.1 pSB1C3-CP29 64.1.2 pSB1C3-CP29 64.1.3 pSB1C3-CP29 64.1.4 pSB1C3-CP29 65.1.1 pSB1C3-CP30 65.1.2 pSB1C3-CP30 65.2.1 pSB1C3-CP30 65.2.2 pSB1C3-CP30 66.5 pSB1C3-CP41 66.6 pSB1C3-CP41 66.7 pSB1C3-CP41 66.8 pSB1C3-CP41 67.1.1 pSB1C3-CP44 67.1.2 pSB1C3-CP44 67.2.1 pSB1C3-CP44 67.2.2 pSB1C3-CP44 *The gel only showed smudgy bands. succesful 47.9 pSB1C3-B0034-STS 47.20 pSB1C3-B0034-STS failed 43. pSB1C3-B0034-TAL 46. pSB1C3-B0034-His-4CL No contamination in negative control -_- Religation from yesterday failed. No colonies, only a few at 76, both red and white. <h2>ther experiments</h2> Contamination control: CA * *Contamination control of growth medium for Lc.lactis and Lb.reuteri. Two test from both bottles. E.coli growth test: CA * *To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </div>';

+

}

+

else if(id == 'd201371')

+

{

+

ds = '<div id="dairy-text"><h1>Monday 2013-07-01</h1>Name of participants: Christoffer, Nils, Magnus, Alexander, Anders Edlund (AE), '+

+

'Mikael Strandgren (MS), Christoffer Ahlström (CA), Viktor Blomkvist (VB), Viktor Törnblom(VT), Nafisa Bashir (NB), Anton Berglund (AB),'+

+

'Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C '+

+

'<h2>Ongoing constructs:</h2>'+

+

'L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP'+

+

' 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) '+

+

'L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid '+

+

'E. faecalis: 9. JH2-2 pAMβ1 '+

+

'L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

'E.coli (strain D5α): 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 42. pSB4S15-red 43. pSB1C3-B0034-TAL '+

+

'44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS '+

+

'60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 '+

+

'67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP 71. pSB3K3-CP29-B0032-BFP'+

+

' 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15'+

+

' Lb. delbrueckii bulgaricus: 18. Lb. delbrueckii bulgaricus '+

+

'L. thermophilus: 19. L.thermophilus '+

+

'<h2>Todays work:</h2>PCR of Crt E, Crt Z, 19, 20, 21, Idi Gelelectroforisis of Crt E, Crt Z, Idi Transformation of 75, 76, 78, 58'+

+

' Subcloning of IspA ->58 Digest of Idi Ligation of 76, 78 O/N: Lb13. Lc. lactis MG1363 pJP059 clone 3 '+

+

'Lb13. Lc. lactis MG1363 pJP059 clone 4 Lb15. Lc. lactis MG1363 pGus clone 3 Lb15. Lc. lactis MG1363 pGus clone 4 7. pSB3K3-red '+

+

'Frozen stock: Lb5.2 plantarum 256 pAMβ1 Lb9. faecalis JH2-2 pAMβ1 clone 4 1. pSB1C3-red 60.PK401 dam- '+

+

'Re-streak: Lb9. faecalis JH2-2 pAMβ1 Lb12. reuteri DSM20016 no plasmid Plasmid preparation: '+

+

'Lb5.2 plantarum 256 pAMβ1 Lb9. faecalis JH2-2 pAMβ1 clone 4 1. pSB1C3-red Assembly: 61. pSB1C3-CP1 '+

+

'62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red '+

+

'Transformation: 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 '+

+

'67. pSB1C3-Cp44 77. pSB4C15-red* '+

+

'*We made a transformation with one sample of 77-digest from today, and one from yesterday to try to see if the problem yesterday laid with the '+

+

'assembly or with the transformation. PCR: 6 colonies of DSM 20016 strain reuteri* 4 colonies of MG1363 strain lactis* '+

+

'*The PCR was carried out with the CBP primers for reuteri and the usp45 primers for lactis. This in order to isolate the signal peptides from each species. '+

+

'45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS '+

+

' Digest: Lb9.4 faecalis JH2-2 pAMβ1 (To rule out chromosomal contamination in prep) Gel electrophoresis: '+

+

'Lb9.4 faecalis JH2-2 pAMβ1 CBP amplicon from reuteri DSM 20016 usp45 amplicon from lactis strain MG1363 45. pSB1C3-B0034-4CL '+

+

'46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS '+

+

'Streaking of yogurt culture: 18. Lb. delbrueckii bulgaricus 19. Lactococcus Thermophilus '+

+

'<h2>Results:</h2>Gel of Crt E and Crt Z was failed at Ethylium Bromide bath. Redo gel Gel of Idi showed succes, proced to subclone. '+

+

'Subclone of IspA showed only red colonies. IspA was incorrectly digested with E instead of X. '+

+

'PCR with good results: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL (Note: weak band) 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS '+

+

'<h2>Other experiments:</h2> </div>';

}

else if(id == 'd201374')

@@ Line 319: / Line 319: @@
   {
    ds = '<div id="dairy-text"><h1>Friday 2013-07-05</h1><br><b>Name of participants:</b> Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A., Christoffer Ahlström (CA), Anton Berglund (AB), Stephanie Herman (SH), Mikael Strandgren (MS), Viktor Blomkvist (VB), Nafisa Bashir (NB), Magnus, Thorsteinn, Alexander, Nils, Christoffer<br><br><h2>Ongoing constructs:</h2><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-CP44<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br><br><b>L. reuteri</b><br>Lb4. 100-23 no plasmid<br>Lb12. DSM 20016 no plasmid<br><br><b>L. plantarum:</b><br>Lb10. 256 no plasmid<br>Lb11. 36E no plasmid<br><br><b>L. thermophilus:</b><br>Lb19. L.thermophilus<br><br><b>Todays work</b><br>Screening PCR & gel analysis of constructs:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br><b>Plasmid prep & glycerol stock:</b><br>48. pSB1C3-B0034-His-STS<br><br><b>O/N:</b> <br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>47.(9, 20) pSB1C3-B0034-STS<br>Lb4. reuteri 100-23 no plasmid<br>Lb10. plantarum 256 no plasmid<br>Lb11. plantarum 36E no plasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Re-streaks:</b><br>Lb19. L.thermophilus<br><br><h2>Results</h2><br>The O/N on 42. pSB4S15-red was done improperly in 15 ml falcon tubes and the lids were screwed on too tightly. A new O/N was done on 42. pSB4S15-red by NB.<br><br><b>PCR screen and gel electrophoresis:</b><br>61.1 pSB1C3-CP1<br>61.2 pSB1C3-CP1<br>62.1 pSB1C3-CP8<br>62.2 pSB1C3-CP8<br>62.3 pSB1C3-CP8<br>62.4 pSB1C3-CP8<br>63.1 pSB1C3-CP11<br>63.2 pSB1C3-CP11<br>63.3 pSB1C3-CP11<br>63.4 pSB1C3-CP11<br>64.1.1 pSB1C3-CP29<br>64.1.2 pSB1C3-CP29<br>64.1.3 pSB1C3-CP29<br>64.1.4 pSB1C3-CP29<br>65.1.1 pSB1C3-CP30<br>65.1.2 pSB1C3-CP30<br>65.2.1 pSB1C3-CP30<br>65.2.2 pSB1C3-CP30<br>66.5 pSB1C3-CP41<br>66.6 pSB1C3-CP41<br>66.7 pSB1C3-CP41<br>66.8 pSB1C3-CP41<br>67.1.1 pSB1C3-CP44<br>67.1.2 pSB1C3-CP44<br>67.2.1 pSB1C3-CP44<br>67.2.2 pSB1C3-CP44<br><br>*The gel only showed smudgy bands.<br><br><b>succesful</b><br>47.9 pSB1C3-B0034-STS<br>47.20 pSB1C3-B0034-STS<br><br><b>failed</b><br>43. pSB1C3-B0034-TAL<br>46. pSB1C3-B0034-His-4CL<br><br>No contamination in negative control -_-<br>Religation from yesterday failed. No colonies, only a few at 76, both red and white. <br><br><h2>ther experiments</h2><br><b>Contamination control:</b> CA *<br><br><i>*Contamination control of growth medium for Lc.lactis and Lb.reuteri.<br> Two test from both bottles. </i><br><br><b>E.coli growth test:</b> CA *<br><br><i>*To see if strain 1 of e.coli in strain database 13 grow on M17 and MRS plates as well. </i></div>';
+ }
+  else if(id == 'd201371')
+ {
+  ds = '<div id="dairy-text"><h1>Monday 2013-07-01</h1><p><b>Name of participants:</b> Christoffer, Nils, Magnus, Alexander, Anders Edlund (AE), <br>'+
+	'Mikael Strandgren (MS), Christoffer Ahlström (CA), Viktor Blomkvist (VB), Viktor Törnblom(VT), Nafisa Bashir (NB), Anton Berglund (AB),'+
+	'Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C<br><br>'+
+	'<h2>Ongoing constructs:</h2>'+
+	'<b>L. reuteri:</b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP'+
+	'<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br>'+
+	'<b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
+	'<b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br>'+
+	'<b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
+	'<b>E.coli (strain D5α):</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>42. pSB4S15-red<br>43. pSB1C3-B0034-TAL<br>'+
+	'44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>'+
+	'60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>'+
+	'67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>71. pSB3K3-CP29-B0032-BFP'+
+	'<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15'+
+	'<br><br><b>Lb. delbrueckii bulgaricus:</b><br>18. Lb. delbrueckii bulgaricus<br><br>'+
+	'<b>L. thermophilus:</b><br>19. L.thermophilus<br><br>'+
+	'<h2>Todays work:</h2>PCR of Crt E, Crt Z, 19, 20, 21, Idi<br>Gelelectroforisis of Crt E, Crt Z, Idi<br>Transformation of 75, 76, 78, 58'+
+	'<br>Subcloning of IspA ->58<br>Digest of Idi<br>Ligation of 76, 78<br><br><b>O/N:</b><br>Lb13. Lc. lactis MG1363 pJP059 clone 3<br>'+
+	'Lb13. Lc. lactis MG1363 pJP059 clone 4<br>Lb15. Lc. lactis MG1363 pGus clone 3<br>Lb15. Lc. lactis MG1363 pGus clone 4<br>7. pSB3K3-red<br><br>'+
+	'<b>Frozen stock: </b><br>Lb5.2 plantarum 256 pAMβ1<br>Lb9. faecalis JH2-2 pAMβ1 clone 4<br>1. pSB1C3-red<br>60.PK401 dam-<br><br>'+
+	'<b>Re-streak: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>Lb12. reuteri DSM20016 no plasmid<br><br><b>Plasmid preparation: </b><br>'+
+	'Lb5.2 plantarum 256 pAMβ1<br>Lb9. faecalis JH2-2 pAMβ1 clone 4<br>1. pSB1C3-red<br><br><b>Assembly: </b><br>61. pSB1C3-CP1<br>'+
+	'62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br>'+
+	'<b>Transformation: </b><br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>'+
+	'67. pSB1C3-Cp44<br>77. pSB4C15-red*<br>'+
+	'*We made a transformation with one sample of 77-digest from today, and one from yesterday to try to see if the problem yesterday laid with the<br>'+
+	'assembly or with the transformation.<br><br><b>PCR: </b><br>6 colonies of DSM 20016 strain reuteri*<br>4 colonies of MG1363 strain lactis*<br>'+
+	'*The PCR was carried out with the CBP primers for reuteri and the usp45 primers for lactis. This in order to isolate the signal peptides from each species.<br>'+
+	'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>'+
+	'<br><b>Digest: </b><br>Lb9.4 faecalis JH2-2 pAMβ1 (To rule out chromosomal contamination in prep)<br><br><b>Gel electrophoresis: </b><br>'+
+	'Lb9.4 faecalis JH2-2 pAMβ1<br>CBP amplicon from reuteri DSM 20016<br>usp45 amplicon from lactis strain MG1363<br>45. pSB1C3-B0034-4CL<br>'+
+	'46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br>'+
+	'<b>Streaking of yogurt culture: </b><br>18. Lb. delbrueckii bulgaricus<br>19. Lactococcus Thermophilus<br><br>'+
+	'<h2>Results:</h2>Gel of Crt E and Crt Z was failed at Ethylium Bromide bath. Redo gel<br>Gel of Idi showed succes, proced to subclone.<br>'+
+	'Subclone of IspA showed only red colonies. IspA was incorrectly digested with E instead of X.<br><br>'+
+	'<b>PCR with good results:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL (Note: weak band)<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br>'+
+'<h2>Other experiments:</h2><br><br></p></div>';
   }
    else if(id == 'd201374')

Template:Team:Uppsala/JS/notebook

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Revision as of 16:39, 26 September 2013