Team:NTU Taiwan/index.html

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                         <div>2013 © NTU-Taiwan.</div>

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                         <div>Produced by <br> <a href="mailto:b99902006@ntu.edu.tw" target="_blank">PoHsien Wang</a></div><br>

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                In Taiwan, fish farmers lose a large amount of fish, because temperature falls dramatically when cold current comes in winter. Ofcourse, fish farmers try to prevent fish from death dying; however, the current methods do not work well. Moreover, they cause damage to the environment. In 2013 iGEM competition, NTU-Taiwan team tries to make a bio-heating device. We transform the SrUCP (uncoupling protein) into yeast. UCP is thermogenic protein which can produce heat by interacting with the electron transport chain. By designing the gene circuit, we want to well control the power of the bio-heating device. In addition, we want to simulate the pound environment in reality by computer and the test results after using our device in low temperature.

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                 Mitochondria uncoupling proteins 1(UCP1) are members of the family of mitochondrial anion carrier proteins (MACP). It facilitates the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. [2][3]UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak.

            UCP has been found in several species, including mammals and plants. Skunk cabbage(Symplocarpus foetidus)is a thermogenic plant, using srUCPs to resist in dramatic temperature decreases in the environment. In 1999, Dr. Kikukatsu Ito found that there are two types of SrUCPs, designated as SrUCPA and SrUCPB [4]. SrUCPA is proposed to be the functional thermogenic prtein, and SrUCPB serves as a regulator in thermogenesis in Skunk cabbage.[5][6] We use SrUCPA gene as our material to construct our plasmid.

 

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                <b><h2>Conference with UT_Tokyo Team</h2></b><br/>

                On 4th May, We held a video conference with UT_Tokyo team. It's our first time to have video conference with foreign iGEM team! We gave a briefly project introduction and discussed about experimental problems to each other. By the discussion, we found out some bugs but some new ideas in our project. On the other hand, we also gave some advices to UT_Tokyo team. That's a great chance for us to practice how can we express our project entirely and clearly.  

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                <b><h2>Conference in Tokyo University</h2></b><br/>

                On 14th July, Yi-Yuan Lee met UT_Tokyo iGEM team in Tokyo University. UT_Tokyo team briefly performed their currently progress and started to discuss their project. For Yi-Yuan, he learned more experience about how to well organitze an iGEM team.

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                <b><h2>Conference with Purdue iGEM Team</h2></b><br/>

                We had a video conference with Purdue iGEM team, Manchester iGEM team, TU Eindhoven iGEM team, and Kyoto iGEM team on July 8th. This is Purdue iGEM team&rsquo;s project. They want to build up a new standardized form for BioBricks register by cooperating and discussing with worldwide iGEM teams. We complete the questionnaire and gave them some advices.

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                <b><h2>Conference in Chiao Tung University</h2></b><br/>

                iGEM NTU_Taiwan team participated in the conference which was hold by Chiao Tung Univeristy. There were 7 teams joined this ceonference, the iGEM team from China, Hong Kong, and most of the iGEM teams in Taiwan. We followed the rule of iGEM which has 20 minute presentation and followed by Q & A time.<br/>

                By this chance, we made friends with iGEMers from different teams and learned a lot of experience from them. After the conference, we had a short trip in Taipei. That was really a great time.

            <h1 class="header">NTU Azalea Festival</h1>

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                NTU Azalea Festival is a anneal event for introducing each department to senior high school students. By this chance, we wanted to spread the iGEM idea to high school students. We design a simple computer game which contain the concept of synthetic biology, molecular biology and bio-Technology. First, we asked them to choose a right enzyme to digest the glactose. And in second level, we asked them to make the enzyme they used in previous level by amino acids, right RNA, and right enzyme. In the last level, we asked them to build up a operon for galactase production.

 

                    <p>Every year, graduate students have poster presentation in the college of life science. In this year, NTU_Taiwan team also participated in the poster presentation and introduced what is iGEM competition to the students in our college.<br/></p>

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            <h1 class="header">Cooperation</h1>

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                    Collaborate with Perdue iGEM team: <br/><br/>

 

                <li>Kyoto iGEM team: <br/><br/>

                            <p>We help Kyoto iGEM team to charaterise their parts. They sent 13 parts to us. We keep discussing how to design the construction and which gene should we use in the characterisation.</p>

 

 

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                 <p class="header"><i>Saccharomyces cerevisiae</i></p>

                 <p>The yeast <b><i>Saccharomyces cerevisiae</i></b> has several properties which have established it as an important tool in the expression of foreign protein for research, industrial or medical use. As a food organism, it is highly acceptable for the production of pharmaceutical proteins. In contrast, <b><i>Escherichia coli</i></b> have toxic cell wall pyroxenes and mammalian cells may contain oncogenic or viral DNA, so that products from these organisms must be tested hmore extensively.</p>

                 <p>Yeast can be grown rapidly on simple media and to high cell density and its genetics are more advanced than any other eukaryote, so that it can be manipulated almost as readily as <b><i>E.coli</i></b>. As a eukaryote, yeast is a suitable host organism for the High-level production of secreted as well as soluble cytosolic proteins.</p>

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                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspThe increasing cost of vegetable oils is turning the use of microbial lipids into a competitive alternative for the production of biodiesel fuel. The oleaginous yeast <i><u>Rhodotorula glutinis</u></i> is able to use a broad range of carbon sources for lipid production, and is able to resist some of the inhibitors commonly released during hydrolysis of lignocellulose materials. <br/>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspSo we choose R.g. for the Expression vector.<br/>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspThermogenic yeast relies on cultural media to grow and to generate heat. This way, the Yeastherm is kind of like burning media as fuel, which is expensive compared with other heating methods such as gas, coal, or electricity. To make our project a more competitive choice when considering large-scale heat production such as heating up a pound in the winter, it is necessary to reduce the cost of culturing yeast. Thanks to previous study in the field of biofuel, we have several solutions of cheaper substitutions of cultural medium contents.<br/>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAccording to Jie Tao and his colleague’s study, agricultural and forestry residues can be used as an alternative of carbon source, taking advantage of <i><u>Rhodotorula glutinis’s</u></i> ability to assimilate xylose.  Agricultural residues such as rice straw and corn stalk are usually burned after harvest. Using these materials not only lower the expense but also benefits the environment. Raw materials like rice or wheat straw are first cut into pieces of appropriate size. They are then hydrolyzed using sulfuric acid with boiling water bath, turninig into hemicellulosic hydrolyzate. Saccharides can be obtained in supernatant after centrifugation and washings of the residue with hot water. <br/>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAfter expressing SrUCP in <i><u>Rhodotorula glutinis</u></i>, we will test for appropriate concentration for Agricultural residue extraction considering growth condition and heating power. The optimized heat power will then be used in large scale simulation as well as calculation of cost reduced. If time permitted, we will dig further into the component of the hemicellulosic hydrolyzate, as it is reviewed that there might be inhibiting compound.<br/>