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Index.Transformation
From 2013.igem.org
(Difference between revisions)
(Created page with "<p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> Mix the two component...") |
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| - | <p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular. Then refresh the competent cells in the 0℃ icy water. <br> | + | <p>1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular.Then refresh the competent cells in the 0℃ icy water. <br> |
| - | Mix the two components and put the EP tube in the icy water for half an hour.<br> | + | 3.Mix the two components and put the EP tube in the icy water for half an hour.<br> |
| - | Next put them in the 42℃ water for 90 secs.<br> | + | 3.Next put them in the 42℃ water for 90 secs.<br> |
| - | Inject the LB solution into the EP tube and culture them at least 40 mins.<br> | + | 4.Inject the LB solution into the EP tube and culture them at least 40 mins.<br> |
| - | Paste them in the plate. | + | 5.Paste them in the plate. |
Latest revision as of 18:17, 26 September 2013
1.Preparation for the competent cells in the -80’c refrigerator, and target DNA molecular.Then refresh the competent cells in the 0℃ icy water.
3.Mix the two components and put the EP tube in the icy water for half an hour.
3.Next put them in the 42℃ water for 90 secs.
4.Inject the LB solution into the EP tube and culture them at least 40 mins.
5.Paste them in the plate.
