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Team:NJU China/Notebook
From 2013.igem.org
(Difference between revisions)
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<span>We learnt to extract RNA from exosomes and cells.</span> | <span>We learnt to extract RNA from exosomes and cells.</span> | ||
<span>We learnt to do RT-PCR and qPCR.</span> | <span>We learnt to do RT-PCR and qPCR.</span> | ||
| - | + | </h3> | |
| - | + | </div> | |
| - | + | </div> | |
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| - | + | <div class="ss-row ss-medium"> | |
| - | + | <div class="ss-left"> | |
| - | + | <h3> | |
| - | + | <a>WEEK 2</a> | |
| - | + | <span>14th-18th, April</span> | |
| - | + | </h3> | |
| - | + | </div> | |
| - | + | <div class="ss-right"> | |
| + | <h3> | ||
<a>siRNA screening (Failed)</a> | <a>siRNA screening (Failed)</a> | ||
| - | <span>14th: We extracted plasmids and cultured the 293t cells.</ | + | <span> |
| - | + | 14th: We extracted plasmids and cultured the 293t cells.</br> | |
| - | + | 15th: We transfected plasmids into 293t cells.</br> | |
| - | + | 16th: We collected cells and preserved it in Trizol.</br> | |
| - | + | 17th: We extracted RNA, then did RT-PCR with the RNA.</br> | |
| - | + | 18th: We did qPCR with the cDNA we got on 17th April.</br> | |
| - | + | 21th: We reexamined the concentration of RNA, and redid qPCR. | |
| - | + | </span> | |
| + | </h3> | ||
| + | </div> | ||
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<div class="ss-row ss-medium"> | <div class="ss-row ss-medium"> | ||
<div class="ss-left"> | <div class="ss-left"> | ||
Revision as of 18:49, 26 September 2013
