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Team:NJU China/Notebook
From 2013.igem.org
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| - | + | <h3> | |
| - | + | <a>WEEK 2</a> | |
| - | + | <span>14th-18th, April</span> | |
| - | + | </h3> | |
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<a>siRNA screening (Failed)</a> | <a>siRNA screening (Failed)</a> | ||
| - | <span>14th: We extracted plasmids and cultured the 293t cells.</ | + | <span> |
| - | + | 14th: We extracted plasmids and cultured the 293t cells.</br> | |
| - | + | 15th: We transfected plasmids into 293t cells.</br> | |
| - | + | 16th: We collected cells and preserved it in Trizol.</br> | |
| - | + | 17th: We extracted RNA, then did RT-PCR with the RNA.</br> | |
| - | + | 18th: We did qPCR with the cDNA we got on 17th April.</br> | |
| - | + | 21th: We reexamined the concentration of RNA, and redid qPCR. | |
| - | + | </span> | |
| + | </h3> | ||
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Revision as of 19:07, 26 September 2013
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