Template:Team:Uppsala/JS/notebook

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(Difference between revisions)
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'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
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}
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else if(id == 'd2013628')
 +
{
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  ds = '<div id="dairy-text"><h1>Friday 2013-06-28</h1><p><b>Name of participants:</b>'+
 +
'Christoffer A, Mikael S, Viktor B, Stephanie H, Viktor T, Jens I, Anders E, Lovisa P., Emil M.,'+
 +
' Peter C., Ken B.-A., Karl H., Nils A, Magnus B, Pontus D, Alexander W<br><br>'+
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'<h2>Ongoing constructs:</h2>'+
 +
'<b>L. reuteri:</b><br>'+
 +
'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>'+
 +
'6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>'+
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'14. DSM 20016-pLUL631(B?)<br><br>'+
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'<b>L. plantarum:</b><br>'+
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'5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br>'+
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'<b>L. lactis:</b><br>'+
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'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
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'<b>E.coli:</b><br>'+
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'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL'+
 +
'-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034'+
 +
'-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034'+
 +
'-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034'+
 +
'-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034'+
 +
'-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3'+
 +
'-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3'+
 +
'-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. '+
 +
'pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>ispA<br><br>'+
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'<h2>Todays work</h2>'+
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'<b>Frozen stock:</b><br>'+
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'Lb13. Lc. lactis MG1363 pJP059 clone 2<br>Lb15. Lc. lactis unknown pGus clone 2<br>Lb16. Lc. lactis'+
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' MG1363 noplasmid clone 2<br><br>'+
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'<b>Digest:</b><br>'+
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'25.pSB1C3-CrtY<br><br>'+
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'<b>Transformation:</b><br>'+
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'24.pSB1C3-CrtI<br>23.pSB1C3-CrtB<br><br>'+
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'<b>Plasmid preparation:</b><br>'+
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'Lb13. Lc. lactis MG1363 pJP059 clone 2 “I, II, III(2x1.5 ml culture)”<br>Lb15. Lc. lactis MG1363 '+
 +
'pGus clone 2 “I, II, III(2x1.5 ml culture)”<br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>'+
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'7. pSB3K3-red<br>11. pSB4A15<br><br>'+
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'<b>Re-streaks:</b><br>'+
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'Lb3. reuteri DSM20016 pVS2<br>Lb8. reuteri 100-23 pGT232*<br>Lb11. plantarum 36E<br>Lb13. Lc. lactis'+
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' MG1363 pJP059<br>Lb16. Lc. lactis MG1363 noplasmid<br>*from frozen stock<br><br>'+
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'<b>Streaking of strains:</b><br>'+
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'Lb15. Lc. lactis MG1363 plasmid unknown pGus:<br>Lb18. Lb. delbrueckii bulgaricus (from freeze dried'+
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' yoghurt culture) <br>Lb19. Lc. Thermophilus (from freeze dried yoghurt culture) <br><br>'+
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'<b>O/N of restreak from Erik Gullberg:</b><br>'+
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'42. pSB4S15-red, clones 1 and 2<br><br>'+
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'<b>PCR:</b><br>'+
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'57.B0034-Idi*<br>ispA*<br>19.1**<br>20.1**<br>21.1**<br>* Amplified from D5α-chromosome. <br>'+
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'** Now with DMSO<br><br>'+
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'<b>Gel electrophoresis:</b><br>'+
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'57.B0034-Idi<br>ispA<br>19.1<br>20.1<br>21.1<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>'+
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'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-'+
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'STS<br><br>'+
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'<b>O/N Screening:</b><br>'+
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'19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker'+
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'-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC'+
 +
'-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker'+
 +
'-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-'+
 +
'CrtB-B0034-CrtI-Linker-Zif268<br><br>'+
 +
'<h2>Results</h2>'+
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'<b>Previous day:</b><br>'+
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'<b>Plasmid preparation:</b><br>'+
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'Lb13. Lc. lactis MG1363 pJP059 clone 1: 6.7 ng/µl (run 4)<br>→ Store in freezer, try again '+
 +
'with new protocol <br><br>'+
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'<b>Transformation:</b><br>'+
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'Lb10. plantarum 256 pLUL364: No growth<br>Lb11. plantarum 36E pVS2: No growth<br>Lb12. Lc. lactis '+
 +
'DSM20016-pVS2: No growth<br>→ Try again later with new competent cells<br><br>'+
 +
'<b>Wednesday 26/6:</b><br>'+
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'<b>Streaking of strains:</b><br>'+
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'Lb13. Lc. lactis MG1363 pJP059: Good growth on all concentrations (1x, 5x & 10x) → Re-streak today<br>'+
 +
'Lb15. Lc. lactis MG1363 pGus: No growth → Try again on new plates, possibly a problem with '+
 +
'antibiotics<br>Lb16. Lc. lactis MG1363 no plasmid: Good growth on all concentrations '+
 +
'(1x, 5x & 10x) → Re-streak today<br><br>'+
 +
'<b>Today:</b><br>'+
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'<b>Plasmid preparation:</b><br>'+
 +
'Lb13. Lc. lactis MG1363-pJP059 clone 2 “I”: 15, 8 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 '+
 +
'“II”: 7,5 ng/µl<br>Lb13. Lc. lactis MG1363-pJP059 clone 2 “III(2x1.5 ml culture)”: 48,3 ng/µl <br>'+
 +
'Lb15. Lc. lactis unknown-pGus clone 2 “I”: 7,3 ng/µ<br>Lb15. Lc. lactis pGus clone 2 “II”: 8,1 ng/µl'+
 +
'<br>Lb15. Lc. lactis pGus clone 2 “III(2x1.5 ml culture)”: 48,1 ng/µl<br>→ Mark and store in freezer.<br><br>'+
 +
'<b>Gel electrophoresis:</b><br>'+
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'57.B0034-Idi: hard do decipher results, redo tomorrow <br>ispA: (“)<br>19.1: failed<br>20.1: failed'+
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'<br>21.1: failed<br>43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: '+
 +
'failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>46. pSB1C3-B0034-His-4CL: failed (“)<br>47. '+
 +
'pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br><br>'+
 +
'<h2>Other experiments</h2>'+
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'<b>THMS buffer preparation</b><br>'+
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'<b>Aliquoting of sigma water</b><br>'+
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'<b>Preparation of antibiotic-free LB-agar plates</b><br>'+
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'<b>Dilution of promotor-oligonucleotides from stock to 5 µM:</b><br>'+
 +
'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
 +
'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br>'+
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'<b>Dilution of promotor oligonucleotide from pellet to 100 µM:</b><br>'+
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'usp45_mut_F<br><br>'+
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'<b>Phosphorylation of promotor-oligonucleotides:</b><br>'+
 +
'CP1_F<br>CP1_R<br>CP11_F<br>CP11_R<br>CP8_F<br>CP8_R<br>CP44_F<br>CP44_R<br>CP30_F<br>CP30_R<br>'+
 +
'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br></p></div>';
  }
  }
   else if(id == 'd201373')
   else if(id == 'd201373')

Revision as of 19:31, 26 September 2013

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