Team:Paris Saclay/Labwork

From 2013.igem.org

(Difference between revisions)
Line 135: Line 135:
* Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 [[File:Psright.png|20px]]
* Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 [[File:Psright.png|20px]]
* Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
* Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
 +
 +
07/22/13
 +
* Digestion of Bba_K592009 by SpeI/PstI, Bba_B0015 and Bba_B0017 by PstI/XbaI
 +
* Denaturation of SpeI/PstI and PstI/XbaI used for the digestion of Bba_K592009, Bba_B0015 and Bba_B0017
Line 185: Line 189:
* Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 [[File:Psright.png|20px]]
* Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 [[File:Psright.png|20px]]
* Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
* Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017
 +
 +
07/22/13
 +
* Digestion of Bba_I732017 by EcoRI/SpeI, Bba_B0015 and Bba_B0017 by PstI/XbaI
 +
* Denaturation of EcoRI/SpeI and PstI/XbaI used for the digestion of Bba_I732017, Bba_K502009, Bba_B0015 and Bba_B0017

Revision as of 21:18, 26 September 2013

Labwork Overview Biobricks Protocols Safety

Contents

Labwork overview

Bba_K1155000

07/01/13

  • Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
  • Ligation of PSB1C3 and Pfnr

07/02/13

  • Tranformation of Bba_K1155000 in DH5α Psright.png

07/03/13

  • Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
  • New culture of Bba_K1155000

07/04/13

  • Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
  • Electrophoresis of Colony PCR products Psright.png
  • Stock of Bba_K1155000
  • Culture of Bba_K1155000
  • Extraction of Bba_K1155000 from DH5α
  • Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again

07/05/13

  • Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI Pswrong.png
  • Extraction of Bba_K1155000 from DH5α

07/15/13

  • Sequensis analysis of Bba_K1155000 Psright.png

WE OBTAIN OUR BIOBRICK


Bba_K1155001

07/09/13

  • PCR purification of BphR1
  • Digestion PCR products : BphR1 and digestion of PSB1C3
  • Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3
  • Ligation of BphR1 and PSB1C3

07/10/13

  • Transformation of ligation of PSB1C3 and BphR1 Psright.png

07/11/13

  • Colony PCR of Bba_K1155001 in DH5α to check good insertion of BphR1 in PSB1C3
  • Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3 Psright.png

07/12/13

  • Stock of clones 5, 6 for Bba_K1155001
  • Extraction of clones 5, 6 for Bba_K1155001 from DH5α

07/15/13

  • Digestion of Bba_K1155001 by EcoRI/PstI and SacII to check the size of fragments
  • PCR of digestion of Bba_K1155001

07/16/13

  • Electrophoresis of the digestion of Bba_K1155001 Psright.png

07/17/13

  • Stock of Bba_K1155001
  • Extraction of Bba_K1155001 from DH5α


Bba_K1155002

07/09/13

  • PCR purification of BphA1
  • Digestion PCR products : BphA1 and digestion of PSB1C3
  • Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3
  • Ligation of BphA1 and PSB1C3

07/10/13

  • Transformation of ligation of PSB1C3 and BphA1 Psright.png

07/11/13

  • Colony PCR of Bba_K1155002 in DH5α to check good insertion of BphA1 in PSB1C3
  • Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3 Psright.png

07/12/13

  • Stock of clones 5, 6, 7, 8 for Bba_K1155002
  • Streak of Bba_K1155002
  • Extraction of clones 5, 6, 7, 8 for Bba_K1155002 from DH5α

07/15/13

  • Digestion of Bba_K1155002 by EcoRI/PstI and SacII to check the size of fragments
  • PCR of digestion of Bba_K1155002

07/16/13

  • Electrophoresis of the digestion of Bba_K1155002 Pswrong.png


Bba_K1155003

07/02/13

  • Tranformation of Bba_K592009 in DH5α Psright.png

07/03/13

  • New culture of Bba_K592009

07/05/13

  • Extraction of Bba_K592009 from DH5α

07/08/13

  • Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
  • Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI Psright.png

07/12/13

  • Transformation of Bba_B0015, Bba_B0017 Psright.png
  • Design of RBS-AmilCP oligos

07/15/13

  • Liquid culture of Bba_B0015, Bba_B0017 Psright.png

07/16/13

  • Extraction of Bba_B0015, Bba_B0017 from DH5α
  • Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI

07/18/13

  • PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI

07/19/13

  • Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 Psright.png
  • Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017

07/22/13

  • Digestion of Bba_K592009 by SpeI/PstI, Bba_B0015 and Bba_B0017 by PstI/XbaI
  • Denaturation of SpeI/PstI and PstI/XbaI used for the digestion of Bba_K592009, Bba_B0015 and Bba_B0017


Bba_K1155004

Bba_K1155005

Bba_K1155006

Bba_K1155007

07/02/13

  • Tranformation of Bba_I732017 in DH5α Psright.png

07/03/13

  • New culture of Bba_I732017

07/05/13

  • Extraction of Bba_I732017 from DH5α

07/08/13

  • Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
  • Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI Psright.png

07/10/13

  • Transformation of Bba_I732019 in DH5α Pswrong.png

07/12/13

  • Transformation of Bba_I732019, Bba_B0015, Bba_B0017 Psright.png

07/15/13

  • Liquid culture of Bba_B0015, Bba_B0017, Bba_I732019 Psright.png

07/16/13

  • Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
  • Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI

07/17/13

  • Electrophoresis of digestions of Bba_B0015, Bba_B0017 by EcoRI/PstI Psright.png
  • Electrophoresis of digestions of Bba_I732019 by EcoRI/PstI Pswrong.png


07/18/13

  • PCR of Bba_B0015, Bba_B0017 digested by EcoRI/PstI

07/19/13

  • Electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017 Psright.png
  • Gel purification of the electrophoresis of Colony PCR products : Bba_B0015, BBa_B0017

07/22/13

  • Digestion of Bba_I732017 by EcoRI/SpeI, Bba_B0015 and Bba_B0017 by PstI/XbaI
  • Denaturation of EcoRI/SpeI and PstI/XbaI used for the digestion of Bba_I732017, Bba_K502009, Bba_B0015 and Bba_B0017


Bba_K1155008

Bba_K1155009

07/01/13

  • Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans

07/09/13

  • PCR purification of BphR2
  • Digestion PCR products : BphR2 and digestion of PSB1C3
  • Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3
  • Ligation of BphR2 and PSB1C3

07/10/13

  • Transformation of ligation of PSB1C3 and BphR2 Psright.png

07/11/13

  • Colony PCR of BphR2 in PSB1C3 in DH5α to check good insertion of BphR2 in PSB1C3
  • Electrophoresis of Colony PCR products : BphR2 in PSB1C3 Psright.png

07/12/13

  • Stock of clones 3, 4 for BphR2
  • Streak of BphR2
  • Extraction of clones 3, 4 for BphR2 from DH5α

07/15/13

  • Digestion of BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments
  • PCR of digestion of BphR2 in PSB1C3

07/16/13

  • Electrophoresis of the digestion of BphR2 in PSB1C3 Pswrong.png


PSB3K3

07/09/13

  • Transformation of Bba_J04450 in DH5α Psright.png

07/10/13

  • Mini and maxi preparation of the culture of Bba_J04450 Psright.png

07/11/13

  • Extraction of Bba_J04450 from DH5α
  • Digestion of Bba_J04450 to chek the size for the plasmid
  • Electrophoresis of the digestion of Bba_J04450 Psright.png

07/16/13

  • Digestion of PSB3K3 by EcoRI/PstI
  • Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI Psright.png
  • Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Labwork"