Team:Carnegie Mellon/Project/Results

From 2013.igem.org

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{{:Team:Carnegie_Mellon/Templates/header}}
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[[image:KillerRed RFP Phototoxicity1.png|thumb|600px|center|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a  HBO100 lamp (includes 375 nm LP filter) for 5 hours.]]
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[[file:KillerRed RFP Phototoxicity1.png|thumb|600px|center|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a  HBO100 lamp (includes 375 nm LP filter) for 5 hours.]]
<p align="center">KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br />
<p align="center">KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br />
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KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)
KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)
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[[image:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]]
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[[file:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]]
<p>XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> cloned with <partinfo>BBa_B0034</partinfo> as the RBS and  <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p>
<p>XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> cloned with <partinfo>BBa_B0034</partinfo> as the RBS and  <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p>
<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%">
<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%">

Revision as of 21:42, 26 September 2013

Killer Red


Figure 1: Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.

KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1.
RFP 114% ± 20% (viable cells)/(Normalized RFU)
KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)

Figure 1: Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp

XL10 Ultracompetent cells were transformed with <partinfo>BBa_K1184000</partinfo> cloned with <partinfo>BBa_B0034</partinfo> as the RBS and <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.

Table 1 Tecan Safire2 Parameters
Excitation (nm)585
Emission (nm)610
Excitation bandwidth (nm)10
Emission bandwidth (nm)10
Gain129
Number of reads10
Integration Time (microseconds)40

Table 2 Shows the fluorescence data over time during photobleaching.
Time (minutes)Fluorescence (RFU)
042598
2037616
4033749
6029059
8025680
10021985
12019442
14017031
16015738
18013741
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