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Team:Carnegie Mellon/HostPhageDynamics
From 2013.igem.org
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The purpose of this model is to illustrate the impact that lambda phages have on a population of E. coli cells. The phages contain a sequence coding for either KillerRed (KR) or Red Fluorescent Protein (RFP). RFP is used as the control in this model because it is 1000 times less phototoxic than KR. The model aims to mimic an experiment that was actually performed by our team in the lab. The general procedure for the experiment is as follows: | The purpose of this model is to illustrate the impact that lambda phages have on a population of E. coli cells. The phages contain a sequence coding for either KillerRed (KR) or Red Fluorescent Protein (RFP). RFP is used as the control in this model because it is 1000 times less phototoxic than KR. The model aims to mimic an experiment that was actually performed by our team in the lab. The general procedure for the experiment is as follows: | ||
</p> | </p> | ||
| - | <p> - Prepare up 8 E. coli cultures </p> | + | <p><blockquote> - Prepare up 8 E. coli cultures </blockquote></p> |
| - | <p> - 4 cultures will be infected with phages containing KR </p> | + | <p><blockquote> - 4 cultures will be infected with phages containing KR </blockquote></p> |
| - | <p> - 4 cultures will be infected with phages containing RFP </p> | + | <p><blockquote> - 4 cultures will be infected with phages containing RFP </blockquote></p> |
| - | <p> - We apply the following conditions on both sets of cultures: </p> | + | <p><blockquote> - We apply the following conditions on both sets of cultures: </blockquote></p> |
| - | <p> o Not induced, not exposed </p> | + | <p><blockquote> o Not induced, not exposed </blockquote></p> |
| - | <p> o Induced, not exposed </p> | + | <p><blockquote> o Induced, not exposed </blockquote></p> |
| - | <p> o Not induced, exposed </p> | + | <p><blockquote> o Not induced, exposed </blockquote></p> |
| - | <p> o Induced, exposed </p> | + | <p><blockquote> o Induced, exposed </blockquote></p> |
| - | <p> - Determine how many cells have been killed by plating cultures and counting CFUs </p> | + | <p><blockquote> - Determine how many cells have been killed by plating cultures and counting CFUs </p> |
<b>Clip 1:</b> Demonstrating the Effect of KillerRed<br> | <b>Clip 1:</b> Demonstrating the Effect of KillerRed<br> | ||
<embed src="https://static.igem.org/mediawiki/2013/a/a9/Netlogo_movie1.mov" width="822" height="640"> | <embed src="https://static.igem.org/mediawiki/2013/a/a9/Netlogo_movie1.mov" width="822" height="640"> | ||
</html> | </html> | ||
Revision as of 21:51, 26 September 2013
Phage-Host Dynamics
What is it?
The purpose of this model is to illustrate the impact that lambda phages have on a population of E. coli cells. The phages contain a sequence coding for either KillerRed (KR) or Red Fluorescent Protein (RFP). RFP is used as the control in this model because it is 1000 times less phototoxic than KR. The model aims to mimic an experiment that was actually performed by our team in the lab. The general procedure for the experiment is as follows:
- Prepare up 8 E. coli cultures
- 4 cultures will be infected with phages containing KR
- 4 cultures will be infected with phages containing RFP
- We apply the following conditions on both sets of cultures:
o Not induced, not exposed
o Induced, not exposed
o Not induced, exposed
o Induced, exposed
- Determine how many cells have been killed by plating cultures and counting CFUs Clip 1: Demonstrating the Effect of KillerRed
