Team:Carnegie Mellon/KRModel

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(Created page with "{{:Team:Carnegie_Mellon/Templates/header}} '''Intracellular model''' The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differen...")
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'''Intracellular model'''
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<h1>Intracellular model</h1>
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<p>
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The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differential equations over three stages of a typical photobleaching expeiment: induction at 37ºC, storage at 4ºC to allow for protein maturation, and photobleaching at room temperature. It includes:
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<ul>
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<li>mRNA synthesis and degradation</li>
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<li>KillerRed synthesis, maturation, and degradation</li>
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<li>KillerRed states: singlet, singlet excited, triplet excited, and deactivated</li>
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<li>Superoxide production (by KillerRed)</li>
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<li>Superoxide elimination (by superoxide dismutase)</li></ul>
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<b>Parameters:<b>
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<ul>
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<li>mRNA synthesis: adapted from Kennell and Riezman 1977.</li>
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The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differential equations over three stages of a typical photobleaching expeiment: induction at 37degC, storage at 4degC to allow for protein maturation, and photobleaching at room temperature. It includes:
+
<li>mRNA degradation: Bernstein, Lin, Cohen, and Lin-Chao 2002.</li>
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mRNA synthesis and degradation
+
<li>KillerRed synthesis: from Kennell and Riezman 1977 and our own fluorescence data</li>
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KillerRed synthesis, maturation, and degradation
+
<li>Superoxide production: adapted from Tsien and Waggoner 1995; Song, Hennink, Young, and Tanke 1995; and our own fluorescence data.</li></p>
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+
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KillerRed states: singlet, singlet excited, triplet excited, and deactivated
+
-
 
+
-
Superoxide production (by KillerRed)
+
-
 
+
-
Superoxide elimination (by superoxide dismutase)
+
-
 
+
-
Parameters:
+
-
 
+
-
mRNA synthesis: adapted from Kennell and Riezman 1977.
+
-
 
+
-
mRNA degradation: Bernstein, Lin, Cohen, and Lin-Chao 2002.
+
-
 
+
-
KillerRed synthesis: from Kennell and Riezman 1977 and our own fluorescence data
+
-
 
+
-
Superoxide production: adapted from Tsien and Waggoner 1995; Song, Hennink, Young, and Tanke 1995; and our own fluorescence data.
+

Revision as of 21:57, 26 September 2013

Killer Red



Intracellular model

The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differential equations over three stages of a typical photobleaching expeiment: induction at 37ºC, storage at 4ºC to allow for protein maturation, and photobleaching at room temperature. It includes:

  • mRNA synthesis and degradation
  • KillerRed synthesis, maturation, and degradation
  • KillerRed states: singlet, singlet excited, triplet excited, and deactivated
  • Superoxide production (by KillerRed)
  • Superoxide elimination (by superoxide dismutase)

Parameters:<b>

  • mRNA synthesis: adapted from Kennell and Riezman 1977.
  • mRNA degradation: Bernstein, Lin, Cohen, and Lin-Chao 2002.
  • KillerRed synthesis: from Kennell and Riezman 1977 and our own fluorescence data
  • Superoxide production: adapted from Tsien and Waggoner 1995; Song, Hennink, Young, and Tanke 1995; and our own fluorescence data.
    • </p>

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