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Team:Carnegie Mellon/Project/Results
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[[file:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]] | [[file:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]] | ||
<p>XL10 Ultracompetent cells were transformed with KillerRed (BBa_K1184000) cloned with <partinfo>BBa_B0034</partinfo> as the RBS and <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p> | <p>XL10 Ultracompetent cells were transformed with KillerRed (BBa_K1184000) cloned with <partinfo>BBa_B0034</partinfo> as the RBS and <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p> | ||
| - | <table cellpadding="2" border="1px" cellspacing="0" align="center" width=" | + | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="50%"> |
<caption><p align="justify"><b>Table 1</b> Tecan Safire<sup>2</sup> Parameters</p></caption> | <caption><p align="justify"><b>Table 1</b> Tecan Safire<sup>2</sup> Parameters</p></caption> | ||
<tr><td><b>Excitation (nm)</b></td><td>585</td></tr> | <tr><td><b>Excitation (nm)</b></td><td>585</td></tr> | ||
Revision as of 22:00, 26 September 2013
KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1.
RFP 114% ± 20% (viable cells)/(Normalized RFU)
KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)
XL10 Ultracompetent cells were transformed with KillerRed (BBa_K1184000) cloned with <partinfo>BBa_B0034</partinfo> as the RBS and <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.
| Excitation (nm) | 585 |
| Emission (nm) | 610 |
| Excitation bandwidth (nm) | 10 |
| Emission bandwidth (nm) | 10 |
| Gain | 129 |
| Number of reads | 10 |
| Integration Time (microseconds) | 40 |
| Time (minutes) | Fluorescence (RFU) |
| 0 | 42598 |
| 20 | 37616 |
| 40 | 33749 |
| 60 | 29059 |
| 80 | 25680 |
| 100 | 21985 |
| 120 | 19442 |
| 140 | 17031 |
| 160 | 15738 |
| 180 | 13741 |
