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Team:Freiburg/Project/modeling
From 2013.igem.org
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TestVar = TestVar.replace(/</g,"\n <"); | TestVar = TestVar.replace(/</g,"\n <"); | ||
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w.document.writeln("<h2> The inserted sequence is: </h2>"); | w.document.writeln("<h2> The inserted sequence is: </h2>"); | ||
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<h1>crRNA-Design</h1> | <h1>crRNA-Design</h1> | ||
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<input type="radio" name="rad" value="2"> coding strand | <input type="radio" name="rad" value="2"> coding strand | ||
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<p>This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to | <p>This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to | ||
Revision as of 22:42, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequence for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.
