Team:Washington/FluorescentAnalysisProtocol

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Revision as of 02:31, 27 September 2013

Fluorescent Analysis Protocol

1. Remove 200 ul culture from each well, and pipette to a 96-well black, clear-bottom plate for analysis.
2. GFP expression levels are analyzed on a spectramax M5e plate reader (bottom read) with an excitation of 480 nm and emission at 520nm. Set the plate reader to automix the samples for 5 seconds prior to analysis.
3. GFP expression levels were normalized against cell density by dividing the relative fluorescence unit values by OD600 data.