Team:Washington/GFPExpression

From 2013.igem.org

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6. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish.</br>
6. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish.</br>
7. Align the spacer plate with the outline of the app in the tablet and tape them down securely.</br>
7. Align the spacer plate with the outline of the app in the tablet and tape them down securely.</br>
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8. Incubate the setup in 370 C for 24 hours.</br>
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8. Incubate the setup in 37C for 24 hours.</br>
9. View plates under a blue light illuminator side by side to discern difference.</br>
9. View plates under a blue light illuminator side by side to discern difference.</br>

Revision as of 03:48, 27 September 2013



GFP Expression on Agar Plates
1. Use 15 ml M9 + Casamino Acid Media in a container
2. Add 150 ul of 10X diluted overnight culture of OD600 2-3
3. Mix them well.
4. Transfer 7.5 ml of the inoculated media into two 60 mm petri dish.
5. When plate solidifies, cover one plate with aluminum foil and incubate at 37o C
6. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish.
7. Align the spacer plate with the outline of the app in the tablet and tape them down securely.
8. Incubate the setup in 37C for 24 hours.
9. View plates under a blue light illuminator side by side to discern difference.

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