Team:Shenzhen BGIC 0101/Home

From 2013.igem.org

(Difference between revisions)
Line 156: Line 156:
}
}
ul.accordion li .description h2{
ul.accordion li .description h2{
-
text-transform: uppercase;
+
    text-transform:uppercase;
-
font-style: normal;
+
    font-style:normal;
-
font-weight: bold;
+
    font-weight:bold;
-
letter-spacing: 1px;
+
    letter-spacing:1px;
-
font-size: 25px;
+
    font-size:45px;
-
color: #444;
+
    color:#444;
-
text-align: left;
+
    text-align:left;
-
margin: -15px 0px 0px 20px;
+
    margin:0px 0px 15px 20px;
-
text-shadow: -1px -1px 1px #ccc;
+
    text-shadow:-1px -1px 1px #ccc;
}
}
ul.accordion li .description p{
ul.accordion li .description p{

Revision as of 05:16, 27 September 2013

igem
  • 1All members of our team.
  • 2Nucleotide modification allow users to modify sequence as their wishes by utilizing codon synonomousness.
  • 3We can help you creat a new chromosome.
  • 4Genove can help you design a new pathway.
  • 5test
11 22 33 44 55


Genovo

Genovo is a Computer-Aided Design（CAD） tool to create genome denovo. The current version is 1.2. It consists of 10 plugins belonging to 3 design modules.
The first module, NeoChr, would assist users to grab related genes in different pathways manually, to rewire genes’ relationship logically, and to replace genes with ortholog that score higher. Then it would allow users to define gene order and orientation in DRAP&DROP way, and add chromosome features to build a brand new genome automatically.
The second one , NucleoMod, would optimize the CDS regions and reduce their synthesis difficulty. And it helps design the CRIPSR site so that we can silence the wild type genes, and in the meanwhile, differentiate the wild type and synthesis type when assemling. This module also can erase or create enzyme site locally.
The third one, SegmMan will cut chromosome into pieces with different sizes with Gibson, Goldengate, Homologous adaptors to them so that they are able to be assembled into whole experimentally.
SegmMan, in another level, provide a complementary OLSDesign will guide users to get the chrosome fragments by chip and how to assembly them together.

  • SegmMan

    SegmMan

    SSegmMan will cut chromosome into pieces in different sizes. And it adds fragments with Gibson, Goldengate, Telomere adaptors to them so that they are able to be assembled into whole experimentally.

    more →
  • NucleoMod

    NucMod

    NucleoMod can modify CDS based on synoymous mutation. It has 5 applications. Firstly, NucleoMod is used to design CRISPR sites on NeoChr so that we can silence the wild type genes. Secondly, it can erase specific enzyme sites according to the users' selection. Thirdly, users can create a enzyme site in selected region of specific genes. Fourthly, it can optimize the codon efficiency to incresase the expression level. Finally, it can smash the tandem repeat bases to reduce the synthesis difficulty.

    more →
  • NeoChr

    NeoChr

    NeoChr is used to construct new chromosome. It would assist users to grab related genes in different pathways manually, to rewire genes’ relationship logically$1, and to replace genes with ortholog that score higher$2. Then it would allow users to define gene order and orientation in DRAG&DROP way, and decouple the genes with overlap. In the end, it would add or delete features, such as telomere, loxp sites to build a brand new genome automatically.

    more →

Retrieved from "http://2013.igem.org/Team:Shenzhen_BGIC_0101/Home"