Team:Goettingen/Team/Reporter

Reporter Team

The activity of transcriptional regulators can be easily monitored by expressing a reporter gene downstream of a promoter, containing their binding site. Recently, in Mycobacterium smegmatis, a transcriptional repressor (DarR) was identified that is able to bind to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a screening system for potential drugs interfering with c-di-AMP biofunction. While many gram-positive bacteria require c-di-AMP for their growth, this molecule is not synthesized by the gram-negative bacterium E. coli. Thus, we intend to build a reporter system consisting of the iGEM biobricks in E. coli (Figure 1).

Figure 1a: When there is no c-di-AMP present, the repressor gene DarR cannot bind to its binding site(operator sequence). The transciption starts and leads to the expression of the reporter gene. This reporter gene could be GFP, or other widely used reporter gene.

Figure 1b: When there is c-di-AMP present, the c-di-AMP can act as ligand of the repressor gene DarR, activating tis function. DarR binds to its binding site, blocking the RNA polymerase, and therefore disrupt transcription. Then we are supposed to see no signal or reduced signal.

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