Team:Groningen/Labwork/27 September 2013

From 2013.igem.org

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<h2>Claudio</h2>
<h2>Claudio</h2>
Starch assay was performed on the 6 colonies which were inoculated yesterday.
Starch assay was performed on the 6 colonies which were inoculated yesterday.
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<br>Colony 1 from each transformation didn't show any amylase activity, therefore 100&micro;l were inoculated in 50mL LB.
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<br><img src="https://static.igem.org/mediawiki/2013/1/1d/Starch_assay_BB18_BB22.jpg" width="50%">
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<br>
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<br>Colony PCR was also performed on the same 6 colonies. The pair of primers used was F-hy_spank-NheI and HM14 (annealing temperature 70&deg;C).
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<br>The samples were checked on agarose gel 0.8%.
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<br><img src="https://static.igem.org/mediawiki/2013/3/34/ColonyPCR_Bs_BB18_BB22_26-09-2013.jpg" width="50%">
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<br>The samples showed to be all positive candidates (expected product size ~2400bps).
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<br>
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<br>Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100&micro;l were inoculated in 50mL LB.
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Revision as of 10:06, 27 September 2013

Claudio

Starch assay was performed on the 6 colonies which were inoculated yesterday.


Colony PCR was also performed on the same 6 colonies. The pair of primers used was F-hy_spank-NheI and HM14 (annealing temperature 70°C).
The samples were checked on agarose gel 0.8%.

The samples showed to be all positive candidates (expected product size ~2400bps).

Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100µl were inoculated in 50mL LB.