Team:Groningen/Labwork/27 September 2013

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<br>The samples showed to be all positive candidates (expected product size ~2400bps).
<br>The samples showed to be all positive candidates (expected product size ~2400bps).
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<br>Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100&micro;l were inoculated in 50mL LB.
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<br>Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100&micro;l of O/N liquid culture were inoculated in 50mL LB.
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Revision as of 10:08, 27 September 2013

Claudio

Starch assay was performed on the 6 colonies which were inoculated yesterday.


Colony PCR was also performed on the same 6 colonies. The pair of primers used was F-hy_spank-NheI and HM14 (annealing temperature 70°C).
The samples were checked on agarose gel 0.8%.

The samples showed to be all positive candidates (expected product size ~2400bps).

Colony 1 from each transformation didn't show any amylase activity and show positive results from the Colony PCR screening, therefore 100µl of O/N liquid culture were inoculated in 50mL LB.