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Team:DTU-Denmark/Notebook/11 June 2013
From 2013.igem.org
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==Procedure== | ==Procedure== | ||
[[Team:DTU-Denmark/Methods/PCR|PCR]] | [[Team:DTU-Denmark/Methods/PCR|PCR]] | ||
| + | |||
| + | *7 rxns in duplicates | ||
| + | *mastermix for 15 rxns | ||
| + | |||
| + | {| | ||
| + | ! !!per rxn!!master | ||
| + | |- | ||
| + | |5x HF buffer||10ul||150ul | ||
| + | |- | ||
| + | |MgCl_2||0.5ul||7.5ul | ||
| + | |- | ||
| + | |dNTPs||5ul||75ul | ||
| + | |- | ||
| + | |Phusion polymerase||0.5ul||7.5ul | ||
| + | |- | ||
| + | |Milli Q water||25ul||375ul | ||
| + | |} | ||
| + | *Total 615ul master mix | ||
== Conclusion from today == | == Conclusion from today == | ||
Revision as of 15:39, 25 June 2013
Contents |
main purposes today
helloworld-project
- diluting primers with 1.5 ml milli Q water each
- diluting G-block with 20ul milli Q water
who were in the lab
Procedure
- 7 rxns in duplicates
- mastermix for 15 rxns
| per rxn | master | |
|---|---|---|
| 5x HF buffer | 10ul | 150ul |
| MgCl_2 | 0.5ul | 7.5ul |
| dNTPs | 5ul | 75ul |
| Phusion polymerase | 0.5ul | 7.5ul |
| Milli Q water | 25ul | 375ul |
- Total 615ul master mix
