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Team:Bonn
From 2013.igem.org
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| - | + | Regulating protein activity is important throughout lifescience research. Even though there are several | |
systems available for regulation of proteins all suffer from certain disadvantages. </br> | systems available for regulation of proteins all suffer from certain disadvantages. </br> | ||
<span style="font-style:italic">Aiming to overcome all of these drawbacks we engineer a novel system for | <span style="font-style:italic">Aiming to overcome all of these drawbacks we engineer a novel system for | ||
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| - | Making versatile control of biological machines easily possible for everybody we engineer a system for | + | Making <span style="font-weight:bold">versatile control of biological machines easily possible</span> for everybody we engineer a system for |
| - | light dependend control of protein activity per protein degradation.</br> | + | <span style="font-weight:bold">light dependend control of protein activity</span> per protein degradation.</br> |
| - | Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused | + | Harnessing the <span style="font-weight:bold">ClpXP protease system</span> of prokaryotes enables degradation of a peptid tag (ssrA) fused |
protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is | protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is | ||
activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br> | activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation. </br> | ||
| - | Modelling of the light dependency of protein degradation will even make simple and accurate control of | + | <span style="font-weight:bold">Modelling of the light dependency</span> of protein degradation will even make simple and accurate control of |
protein activity for any desired level of activity possible.</br> | protein activity for any desired level of activity possible.</br> | ||
| - | As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.</br> | + | As a proof of principle we investigate degradation of the <span style="font-weight:bold">fluorescent reporter protein mCherry.</span></br> |
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Revision as of 16:02, 25 June 2013
Regulating protein activity is important throughout lifescience research. Even though there are several
systems available for regulation of proteins all suffer from certain disadvantages.
Aiming to overcome all of these drawbacks we engineer a novel system for
light dependend regulation of protein degradation resulting in a rapid change of protein activity.
Our system can be not only of great importance for fundamental science to investigate protein function but
also for accurate control of bioreactors and biological machines.
Project
Making versatile control of biological machines easily possible for everybody we engineer a system for
light dependend control of protein activity per protein degradation.
Harnessing the ClpXP protease system of prokaryotes enables degradation of a peptid tag (ssrA) fused
protein upon induction with the adaptor protein SspB. Using a Split version of SspB, protein degradation is
activated through heterodimerisation of both SspB parts.We utilize light dependent heterodimerisation to eventually obtain light inducible degradation.
Modelling of the light dependency of protein degradation will even make simple and accurate control of
protein activity for any desired level of activity possible.
As a proof of principle we investigate degradation of the fluorescent reporter protein mCherry.
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Background
To enable protein degradation we decided on one proteases system (ClpXP), which allows specific degradation
of proteins, is highly conserved and well established.
For light dependency we compared several systems to finally find the best suited one. We created a
comprehensive overview of all systems making artificially light control possible so that everyone can
easily choose the optimal system for his approach.
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Human Practice
The major goal of our Human Practice work is to make Synthethic Biology easily understandable and intresting for everybody.
For that purpose we introduce Synthetic Biology and our project to the generald public via developing a comic series, programming a computer game and conduct several presentations in schools all over germany.
Getting the general public even more in touch with science we furthermore planned a Science Slam.
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Team
We are the iGEM team from Bonn consisting of 22 students from the fields Biomedicine, Biology, Biotechnology and Mathematics. Organization and planning of our project is carried out by veternary members, already have been part of the last year iGEM Team. A large portion of our team consists of first year bachelor students making our team highly dynamic, innovative and motivated.
Our lab is part of the Life and Medical Sciences Institute in Bonn.
We would like to thank all of our sponsors for making this project possible.
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