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Team:Groningen/25 June 2013
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(Created page with "A couple of media are prepared: <br/>LB medium with agar 1 liter <br/>LB medium divided into 80 ml portions <br/>70% ethanol <br/>2 liter TBE buffer Both LB media are autoclaved...")
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Revision as of 17:33, 25 June 2013
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.
"B.subtilis" strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for extraction of genomic DNA is arranged.
