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Team:Groningen/25 June 2013
From 2013.igem.org
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Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove. | Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove. | ||
| - | + | ''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for extraction of genomic DNA is arranged. | |
Revision as of 17:34, 25 June 2013
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer
Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.
B. subtilis strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for extraction of genomic DNA is arranged.
