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Team:Groningen/26 June 2013
From 2013.igem.org
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| - | Extraction of the genomic DNA ''B. subtilis''. | + | Extraction of the genomic DNA of ''B. subtilis'' strain 168. |
Diluting the primers (to 100 mM and 10 mM) | Diluting the primers (to 100 mM and 10 mM) | ||
Revision as of 13:28, 26 June 2013
Extraction of the genomic DNA of B. subtilis strain 168.
Diluting the primers (to 100 mM and 10 mM)
Diluting the dNTPs to a concentration of 10 mM.
Run a PCR for the four different signal sequences.
-FliZ
-MotB
-EstA
-LytB
The size will be around 100-150 bp.
The following protocol is used for the PCR of EstA, MotB and LytB:
98°C, 98°C, 50°C, 72°C, 72°C, 4°C
0:30 0:10 0:25 0:05 10:00 forever
The following protocol is used for the PCR of FliZ: 98°C, 98°C, 45°C, 72°C, 72°C, 4°C 0:30 0:10 0:25 0:04 10:00 forever
