Team:UPenn/Notebook

From 2013.igem.org

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</head>
</head>
<body>
<body>
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    <div class="outer">
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    <ul class="nav">
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        <li><a href="https://2013.igem.org/Team:UPenn">Home</a></li>
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        <li><a href="#three">Team</a></li>
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        <li>Official&nbsp;&nbsp;&nbsp;Team&nbsp;&nbsp;&nbsp;&nbsp; Profile</li>
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        <li><a href="https://2013.igem.org/Team:UPenn/Project">Project</a></li>
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        <li>Parts</li>
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        <li>Modeling</li>
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        <li><a href="https://2013.igem.org/Team:UPenn/Notebook">Notebook</a></li>
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        <li>Safety</li>
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     Last year's notebook:
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<p style="text-align:center;color:white;">June 2012 Notebook</p>
<p style="text-align:center;color:white;">June 2012 Notebook</p>
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<li>Finalized synthesis order (minus linker)</li>
<li>Finalized synthesis order (minus linker)</li>
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Revision as of 16:34, 26 June 2013

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

 

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

 

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Retrieved from "http://2013.igem.org/Team:UPenn/Notebook"