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Team:NYMU-Taipei/Modeling/ModSensors
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(Created page with " ==Function of the parts== trxC promoter is an oxyR-activated promoter. We use trxC promoter as a sensor/ switch to have the whole circuit opened when Nosema exists, and closed w...")
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(Created page with " ==Function of the parts== trxC promoter is an oxyR-activated promoter. We use trxC promoter as a sensor/ switch to have the whole circuit opened when Nosema exists, and closed w...")
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Revision as of 16:38, 27 September 2013
Function of the parts
trxC promoter is an oxyR-activated promoter. We use trxC promoter as a sensor/ switch to have the whole circuit opened when Nosema exists, and closed when Nosema is killed. When Nosema enters the bees, it will trigger bees’ ROS (reactive oxygen species) production, which in turn launches E.coli’s OxyR production. After that ROS and OxyR will form a complex and binds to trxC promoter. To increase the sensitivity of this switch, we add an additional circuit expressing OxyR regulated by a constitutive promoter to enhance the basal OxyR expression in E.coli.
