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| - | {{Team:HokkaidoU_Japan/header_Maestro}}
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| - | <html>
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| - | <div id="common-header-bottom-background">
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| - | <div class="wrapper">
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| - | <h1 id="common-header-title">Maestro E.coli</h1>
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| - | <h2 id="common-header-subtitle">Notebook</h2>
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| - | <img id="common-header-img" src="https://static.igem.org/mediawiki/2013/e/ea/HokkaidoU2013_Maestro_Header.png">
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| - | </div>
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| - | </div>
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| - | <div class="wrapper">
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| - | <div id="hokkaidou-contents">
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| - | <!-- end header / begin contents -->
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| - | <h2>R0040,R0010-I732020</h2>
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| - | <div class="workflow-container">
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| - | <ul class="steps">
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| - | <li class="step start">
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| - | <div class="step-tile"><h2>Start</h2></div>
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| - | </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jul 12</time> </li> </ul> <div class="step-recipe"> R0010, R0040 from distribution kit<br> each of 2µL DNA to JM109 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Jul 12</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Jul 13</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Digestion</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 1</time> </li> </ul> <div class="step-recipe"> Cut R0010, R0040 with SpeI, PstI (using 10×H buffer)<br> Cut I732020 with XbaI, PstI (using 10×M buffer) </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Antarctic phosphatase</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Thu Aug 1</time> </li> </ul> <div class="step-recipe"> R0010, R0040 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Ligation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 2</time> </li> </ul> <div class="step-recipe"> Ligate (R0010, R0040) with I732020 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Transformation</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Fri Aug 2</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020<br> each of 2µL DNA to JM109 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Colony PCR & Culture</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sat Aug 3</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020 </div> </div> </div> </li>
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| - | <li class="step"> <div class="step-tile"> <h2 class="step-title">Mini-prep</h2> </div> <div class="step-detail"> <div class="step-detail-body"> <ul class="step-detail-trials"> <li> <time>Sun Aug 4</time> </li> </ul> <div class="step-recipe"> (R0010, R0040)-I732020 </div> </div> </div> </li>
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| - | <li class="step complete">
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| - | <div class="step-tile"><h2>Complete!</h2></div>
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| - | </li>
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| - | </ul>
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| - | </div>
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| - |
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| - | <!-- end contents / begin footer -->
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| - | </div>
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| - | </div>
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| - | </html>
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| - | {{Team:HokkaidoU_Japan/footer}}
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