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Team:Biwako Nagahama/Material & Method
From 2013.igem.org
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= <h2>Material & Method</h2> = | = <h2>Material & Method</h2> = | ||
== <h2>Genaral protocol</h2> == | == <h2>Genaral protocol</h2> == | ||
| + | <h3>Distribution kit</h3> | ||
| + | ---- | ||
| + | <p>↓With a pipette tip, punch a hole in the foil</p> | ||
| + | <p>↓Add 10μL of dH2O,and pipetting</p> | ||
| + | <p>↓Put 5min</p> | ||
| + | <p>↓Pipette 1uL of the resuspended DNA Transformation into your desired 100μL of competent cells</p> | ||
| + | <p>↓Hold on ice for 20min</p> | ||
| + | <p>↓Heat shock at 42℃ for 30sec</p> | ||
| + | <p>↓quickly</p> | ||
| + | <p>↓On ice for 2min</p> | ||
| + | <p>↓Add 900μL of SOCborth</p> | ||
| + | <p>↓Hold at 37℃ for 30min</p> | ||
| + | <p>↓Plating 100μL of DNA Transformation</p> | ||
| + | <p>↓Centrifuge for 1 min(13,000rpm)</p> | ||
| + | <p>↓Waste supernatant for 800μL, and pipetting</p> | ||
| + | <p>↓Plating all</p> | ||
| + | <p>↓Incubate at 37℃ (over night)</p> | ||
== <h2>CelC</h2> == | == <h2>CelC</h2> == | ||
Revision as of 17:29, 27 September 2013
Contents |
Material & Method
Genaral protocol
Distribution kit
↓With a pipette tip, punch a hole in the foil
↓Add 10μL of dH2O,and pipetting
↓Put 5min
↓Pipette 1uL of the resuspended DNA Transformation into your desired 100μL of competent cells
↓Hold on ice for 20min
↓Heat shock at 42℃ for 30sec
↓quickly
↓On ice for 2min
↓Add 900μL of SOCborth
↓Hold at 37℃ for 30min
↓Plating 100μL of DNA Transformation
↓Centrifuge for 1 min(13,000rpm)
↓Waste supernatant for 800μL, and pipetting
↓Plating all
↓Incubate at 37℃ (over night)
