Team:DTU-Denmark/Methods/PCR-touchUp

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# 72°C for 5:00 - Final extension  
# 72°C for 5:00 - Final extension  
# End
# End
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For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link])

Revision as of 20:09, 26 June 2013

Example of the method of touch up PCR

Touch up PCR is a method of amplifying a PCR-product that have bad primers or are troublesome in other ways. This is an example:

  1. 98°C for 2:00 - Denaturing and warm up
  2. 98°C for 0:10 - Denaturing after each cycle
  3. 50°C for 0:30 - Annealing
    • Temperature increment of 1°C/cycle
  4. 72°C for 2:00 - Elongation
  5. Go to number 2 - repeat 10 times
  6. Go to number 2 - repeat 5 times
  7. 72°C for 5:00 - Final extension
  8. End

For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link])

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