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'CP29_F CP29_R CP41_F CP41_R </div>';

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ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1> Name of participants: Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid E.coli: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 23.pSB1C3-CrtB 24.pSB1C3-CrtI 25.pSB1C3-CrtY 42. D5α with plasmid pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS <h2>Todays work</h2> Transformation: Lb11. plantarum 36E pVS2 Lb10. plantarum 256 pLUL634 Lb12. plantarum DSM20016 pVS2 Assembly: 23.Crt.B + pEL3A15 24.Crt.I + pEL3K16 Frozen stock: Lb9. faecalis JH2-2 pAMβ1 Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb16. Lc. lactis MG1363 no plasmid clone 1 Plasmid preparation: Lb13. Lc. lactis MG1363 pJP059 clone 1 Lb15. Lc. lactis MG1363 pGus clone 1 Lb9. faecalis JH2-2 pAMβ1 Over night: 3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 Gel electrophoresis: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 19. (digest from previous day) 20. (“) 21. (“) PCR amplification of construct: miraculin 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25. pSB1C3-CrtY 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS O/N screening PCR: 19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 25.pSB1C3-CrtY <h2>Results</h2> Previous day: O/N: Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! * Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!* Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! ** Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! ** * Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day ** → Frozen stock and plasmid prep. Transformation: 42. pSB4S15-red: Many small white colonies, contamination with cocci? → Discard, get clones from Erik G. Streaking of strains: Lb13. Lc. lactis MG1363 pJP059: Some growth. Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock. Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock. → let grow one more day. Today: Plasmid preparation: Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl Lb13. Lc. lactis MG1363-pJP059 clone 1: 4.9 ng/µl (run 1) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2) Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3) Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1) → Measure again tomorrow Gel electrophoresis: Miraculin: fail, problems with primers, probably due to problematic secondary structure. 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27) 19. (digest from previous day): Success 20. (“): Success 21. (“): Success 19. (PCR): Fail -> redo with lower TM 20. (“): Fail -> redo with lower TM 21. (“): Fail -> redo with lower TM 25. pSB1C3-CrtY: Good results <h2>Other experiments</h2> Preparation of THMS buffer</div>'

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{

ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1> Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A, Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 1. pSB1C3-red 7. pSB3K3-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red L. plantarum: Lb10. 256-noplasmid Lb11. 36E-noplasmid L. reuteri: Lb12. DSM 20016-noplasmid L. lactis: Lb13. MG1363-pJP059 <h2>Todays work</h2> PCR: Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* *Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Lb13.2.2 lactis MG1363 pJP059 (plasmid)** Lb13.2.3 lactis MG1363 pJP059 (plasmid)** Lb13.3 lactis MG1363 pJP059 (colony)** Lb13.4 Lc. lactis MG1363 pJP059 (colony)** **Gel will be done tomorrow. Followed the PCR protocol with DMSO. 78. pEL3C18-CrtY* -*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time) Digest: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Ligation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 1. pSB1C3-red -as N/C, one that we use plus a digest that has worked for other groups 7. pSB3K3-red -as N/C, one that we use plus a digest that has worked for other groups 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red -*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning. Transformation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77 pSB4C15-red O/N: Lb10.1. plantarum 256 no plasmid Lb11.1. plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 Plasmid preparation: 7.pSB3K3 22.pSB1C3-B0032-BFP 42. pSB4S15-red Gelelectrophoresis: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Lb13.2.3 Lc. lactis MG1363-pJP059* Lb13.3 Lc. lactis MG1363-pJP059* Lb13.4 Lc. lactis MG1363-pJP059* *Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Results Gelelectrophoresis: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red -Digestion Worked failed: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* -*The gel showed nothing but primer dimer. Digest: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Yesterdays transformation: successful: 61. pSB1C3-CP1: Growth 61. pSB1C3-CP1 10x: Some growth 62. pSB1C3-CP8: Some growth 62. pSB1C3-CP8 10x: No growth 63. pSB1C3-CP11: Some growth 63. pSB1C3-CP11 10x: No growth 64. pSB1C3-CP29: Some growth 64. pSB1C3-CP29 10x: No growth 65. pSB1C3-CP30: Some growth 65. pSB1C3-CP30 10x: No growth 66. pSB1C3-CP41: Growth 66. pSB1C3-CP41 10x: Some growth 67. pSB1C3-Cp44: No growth 67. pSB1C3-Cp44 10x: No growth 68. pSB3K3-CP1-B0032-BFP: No growth 68. pSB3K3-CP1-B0032-BFP 10x: Growth 69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated 69. pSB3K3-CP8-B0032-BFP 10x: No growth 70. pSB3K3-CP11-B0032-BFP: Growth 70. pSB3K3-CP11-B0032-BFP 10x: Growth 71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies 71. pSB3K3-CP29-B0032-BFP 10x: ----- 72. pSB3K3-CP30-B0032-BFP: Growth 72. pSB3K3-CP30-B0032-BFP 10x: No growth 73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies 73. pSB3K3-CP41-B0032-BFP 10x: No growth 77.1 pSB4C15, clone 1: No growth 77.1 pSB4C15, clone 1 10x: No growth 77.2 pSB4C15, clone 2: Some growth 77.2 pSB4C15, clone 2 10x: No growth 23. pSB1C3-CrtB 24. pSB1C3-CrtI 27. pEL3K16-red 38. pEL3A15-red 51. pEL3C18-red failed: 81. pSB4S15-CP41: No growth 82. pSB4S15-CP29: No growth 83. pSB4S15-CP30: No growth → Redo ligation and transformation. Plasmid preparation: 7.3 pSB3K3 clone 3: 50.1 ng/µl 7. 4 pSB3K3 clone 4: 43.4 ng/µl 7. 5 pSB3K3 clone 5: 48.1 ng/µl 22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl 22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ 22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ 42.1 pSB4S15-red clone 1: 43.6 ng/µl 42.2 pSB4S15-red clone 2: 46.1 ng/µl <h2>Other experiments</h2> Made agarplates with chloramphenicol resistance MRS-broth preparation MRS-broth-agar preparation MRS-broth-agar plates preparation </div>';

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 'CP29_F<br>CP29_R<br>CP41_F<br>CP41_R<br><br></p></div>';
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-  else if(id == 'd201373')
+else if(id == 'd2013627')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-27</h1><br><b>Name of participants: </b>Marcus H, Lovisa P, Emil M, Peter C, Ken B-A, Karl H, Kristoffer L, Christoffer A, Mikael S, Jens I, Nafisa B, Stephanie H, Alona N, Viktor T, Viktor B, Nils, Alexander, Thorsten, Pontus, Magnus, Malin<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br><b>E.coli:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>23.pSB1C3-CrtB<br>24.pSB1C3-CrtI<br>25.pSB1C3-CrtY<br>42. D5α with plasmid pSB4S15-red <br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><h2>Todays work</h2><br><b>Transformation:</b><br>Lb11. plantarum 36E pVS2<br>Lb10. plantarum 256 pLUL634<br>Lb12. plantarum DSM20016 pVS2<br><br><b>Assembly:</b><br>23.Crt.B + pEL3A15<br>24.Crt.I + pEL3K16<br><br><b>Frozen stock: </b><br>Lb9. faecalis JH2-2 pAMβ1<br>Lb13. Lc. lactis MG1363 pJP059 clone 1<br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb16. Lc. lactis MG1363 no plasmid clone 1<br><br><b>Plasmid preparation: </b><br>Lb13. Lc. lactis MG1363 pJP059 clone 1 <br>Lb15. Lc. lactis MG1363 pGus clone 1<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Over night:</b><br>3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br><br><b>Gel electrophoresis:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>19. (digest from previous day)<br>20. (“)<br>21. (“)<br><br><b>PCR amplification of construct:</b><br>miraculin<br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25. pSB1C3-CrtY<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br><br><b>O/N screening PCR:</b><br>19.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>25.pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059 clone 2: Visual pellet, foggy medium; nc ok! * <br>Lb15. Lc. lactis MG1363 pGus clone 2: Visual pellet, foggy medium; nc ok! *<br>Lb16. Lc. lactis MG1363 no plasmid clone 2: Visual pellet, foggy medium; nc ok!*<br>Lb13. Lc. lactis MG1363 pJP059 clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb15. Lc. lactis MG1363 pGus clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>Lb16. Lc. lactis MG1363 no plasmid clone 1: Visual pellet, slightly foggy medium; nc ok! **<br>* Optimized conditions = 10µg/ml antibiotics, fill almost up the falcon tube with M17-broth to get rid of the air, let grow in 30o C, make sure that the bacteria pellet is dissolved. → Let grow for one more day <br>** → Frozen stock and plasmid prep.<br><br><b>Transformation: </b><br>42. pSB4S15-red: Many small white colonies, contamination with cocci?<br>→ Discard, get clones from Erik G.<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059: Some growth.<br>Lb15. Lc. lactis MG1363 pGus: Hard to see without opening lock.<br>Lb16. Lc. lactis MG1363 no plasmid: Hard to see without opening lock.<br>→ let grow one more day.<br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>Lb9. faecalis JH2-2 pAMβ1 clone 1: 10.1 ng/µl<br>Lb9. faecalis JH2-2 pAMβ1 clone 2: 24.7 ng/µl<br>Lb13. Lc. lactis  MG1363-pJP059 clone 1: 4.9 ng/µl (run 1)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.0 ng/µl (run 2)<br>Lb13. Lc. lactis MG1363-pJP059 clone 1: 6.1 ng/µl (run 3)<br>Lb15. Lc. lactis MG1363 pGus clone 1: 61 ng/µl (run 1)<br>→ Measure again tomorrow<br><br><b>Gel electrophoresis:</b><br>Miraculin: fail, problems with primers, probably due to problematic secondary structure.<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: failed, ladder too, probably problem with gel. Image 1 (2013.06.27)<br>19. (digest from previous day): Success<br>20. (“): Success<br>21. (“): Success<br>19. (PCR): Fail -> redo with lower TM<br>20. (“): Fail -> redo with lower TM<br>21. (“): Fail -> redo with lower TM<br>25. pSB1C3-CrtY: Good results<br><br><h2>Other experiments</h2><br><b>Preparation of THMS buffer</b></div>'
+ }
+ else if(id == 'd201373')
   {
    ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1><br><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A,  Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>1. pSB1C3-red<br>7. pSB3K3-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br><b>L. plantarum:</b><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br><br><br><b>L. reuteri:</b><br>Lb12. DSM 20016-noplasmid<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br><br><h2>Todays work</h2><br><b>PCR:</b><br>Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	  (colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	  (colony)*<br><i>*Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br>Lb13.2.2 lactis MG1363 pJP059	(plasmid)**<br>Lb13.2.3 lactis MG1363 pJP059	(plasmid)**<br>Lb13.3 lactis MG1363 pJP059		(colony)**<br>Lb13.4 Lc. lactis MG1363 pJP059	(colony)**<br><i>**Gel will be done tomorrow. Followed the PCR protocol with DMSO.</i><br>78. pEL3C18-CrtY*<br><i>-*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time)</i><br><br>Digest:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Ligation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>1. pSB1C3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>7. pSB3K3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><i>-*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning.</i><br><br><b>Transformation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77 pSB4C15-red<br><br><b>O/N:</b><br>Lb10.1. plantarum 256 no plasmid<br>Lb11.1. plantarum 36E no plasmid <br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br><br><b>Plasmid preparation:</b><br>7.pSB3K3<br>22.pSB1C3-B0032-BFP<br>42. pSB4S15-red<br><br><b>Gelelectrophoresis:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br>Lb13.2.3 Lc. lactis MG1363-pJP059*<br>Lb13.3 Lc. lactis MG1363-pJP059*<br>Lb13.4 Lc. lactis MG1363-pJP059*<br><i>*Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br><br><b>Results</b><br><b>Gelelectrophoresis:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><i>-Digestion Worked</i><br><br><b>failed:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	(colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	(colony)*<br><i>-*The gel showed nothing but primer dimer.</i><br><br><b>Digest:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Yesterdays transformation:</b><br><b>successful:</b><br>61. pSB1C3-CP1: Growth<br>61. pSB1C3-CP1 10x: Some growth<br>62. pSB1C3-CP8: Some growth<br>62. pSB1C3-CP8 10x: No growth<br>63. pSB1C3-CP11: Some growth<br>63. pSB1C3-CP11 10x: No growth<br>64. pSB1C3-CP29: Some growth<br>64. pSB1C3-CP29 10x: No growth<br>65. pSB1C3-CP30: Some growth<br>65. pSB1C3-CP30 10x: No growth<br>66. pSB1C3-CP41: Growth<br>66. pSB1C3-CP41 10x: Some growth<br>67. pSB1C3-Cp44: No growth<br>67. pSB1C3-Cp44 10x: No growth<br>68. pSB3K3-CP1-B0032-BFP: No growth<br>68. pSB3K3-CP1-B0032-BFP 10x: Growth<br>69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated<br>69. pSB3K3-CP8-B0032-BFP 10x: No growth<br>70. pSB3K3-CP11-B0032-BFP: Growth<br>70. pSB3K3-CP11-B0032-BFP 10x:  Growth<br>71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies<br>71. pSB3K3-CP29-B0032-BFP 10x: -----<br>72. pSB3K3-CP30-B0032-BFP: Growth<br>72. pSB3K3-CP30-B0032-BFP 10x: No growth<br>73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies<br>73. pSB3K3-CP41-B0032-BFP 10x: No growth<br>77.1 pSB4C15, clone 1: No growth<br>77.1 pSB4C15, clone 1 10x: No growth<br>77.2 pSB4C15, clone 2: Some  growth<br>77.2 pSB4C15, clone 2 10x: No growth<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>27. pEL3K16-red<br>38. pEL3A15-red<br>51. pEL3C18-red<br><br><b>failed:</b><br>81. pSB4S15-CP41: No growth<br>82. pSB4S15-CP29: No growth<br>83. pSB4S15-CP30: No growth<br><i>→ Redo ligation and transformation.</i><br><br><b>Plasmid preparation:</b><br>7.3  pSB3K3 clone 3: 50.1 ng/µl<br>7. 4 pSB3K3 clone 4: 43.4 ng/µl<br>7. 5 pSB3K3 clone 5: 48.1 ng/µl<br>22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl<br>22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ<br>22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ<br>42.1 pSB4S15-red clone 1: 43.6 ng/µl<br>42.2 pSB4S15-red clone 2: 46.1 ng/µl<br><br><h2>Other experiments</h2><br>Made agarplates with chloramphenicol resistance<br>MRS-broth preparation<br>MRS-broth-agar preparation <br>MRS-broth-agar plates preparation </div>';

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