Team:Berkeley/Safety

**Safety forms were approved on 9/18/13 by David Lloyd and Julie McNamara.



Our team worked only with the Escherichia Coli and Saccharomyces Cerevisiae species. E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections (http://epa.gov/oppt/biotech/pubs/fra/fra004.htm). S.cerevisiae is a GRAS organism (Generally recognized as Safe). "Saccharomyces, as a genus, present low risk to human health or the environment. Criteria used to differentiate between species are based on their ability to utilize specific carbohydrates without relevance to pathogenicity."
(http://www.epa.gov/biotech_rule/pubs/fra/fra002.htm).

2) Highest Risk Group Listed: 1

3) List and describe all new or modified coding regions you will be using in your project. (If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)

• Part name: BBa_K1131000
  
• Where did you get the physical DNA for this part (which lab, synthesis company, etc): Synthesized, IDT
  
• What species does this part originally come from?: Methylophaga aminisulfidivorans
  
• What is the Risk Group of the species?: 1
  
• What is the function of this part, in its parent species?: Flavin-containing monooxygenase which hydroxylates indole.
  

• Part name: BBa_K1131001_Glu
  
• Where did you get the physical DNA for this part (which lab, synthesis company, etc): Synthesized, IDT
  
• What species does this part originally come from?: Bacillus circulans
  
• What is the Risk Group of the species?: 1
  
• What is the function of this part, in its parent species?: Beta-Glucosidase active on indican to produce indoxyl.
  

4) Do the biological materials used in your lab work pose any of the following risks? Please describe.

• Risks to the safety and health of team members or others working in the lab?
  
  No known health risks posed by the materials used. Preventive measures such as PPE (Personal Protective Equipment) are in place to avoid any unforeseen risks.
  
• Risks to the safety and health of the general public, if released by design or by accident?
  
  No known health risks posed by the materials used. Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks.
  
• Risks to the environment, if released by design or by accident?
  
  No known environmental risks posed by the materials used. All materials utilized have been studied extensively and are considered safe by all competent agencies. Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks.
  
• Risks to security through malicious misuse by individuals, groups, or countries?
  
  No known security risks posed by the materials used. All materials utilized have been studied extensively and are considered safe by all competent agencies. Preventive measures such as sterilization of materials prior to disposal are in place to avoid any unforeseen risks.