Team:Korea U Seoul/Project/sub proc result
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Revision as of 21:09, 27 September 2013
CaCO3 Precipitation Assay
Method
The overall reaction for precipitation isThe effect of NA and R on the rate of precipitation of CaCO3 was determined by recording the decrease in pH of a 20mM Tris HCl pH8.0 buffer solution at 10°C
Reagents
- -20mM Tris-HCl buffer, pH 8.0.
- -20mM Tris-HCl buffer, pH 8.0 + 1M Imidazole.
- -1M NaHCO3.
- -1M CaCl2.
Enzyme
Using Protino® Ni-NTA Columns for His-tag protein purification, purify the enzymes.These enzymes is diluted in 20mM Tris-HCl buffer, pH 8.0 + 10mM Immidazole at 4°C.