"
Team:Korea U Seoul/hp/safty Q
From 2013.igem.org
(Difference between revisions)
(Created page with "<html> <head> <style> h2 { margin-bottom: 30px} p > img { float: right; width: 300px; margin: 10px;} .content_ {overflow: auto;} ...") |
|||
| Line 15: | Line 15: | ||
<div class="a"> | <div class="a"> | ||
<ol type="A"> | <ol type="A"> | ||
| - | <li> | + | <li>Issues to the researcher safety</li> |
<ol> | <ol> | ||
| - | <li type="1"> | + | <li type="1">E.coli(BW25113, DH5a, BL21(DE3)</li> |
Moreover, E. coli strain we are using are BW25113, DH5a and BL21(DE3). Those E. colis are used for produce proteins(nacrein) and they use different promoters. Refer to former research they studied widely about safety and by the result founded to be very safe. They also weakened for laboratory use. Therefore would not pose a threat to environment. Thus, this E. coli strain is less likely to harm researchers. <br /><br /> | Moreover, E. coli strain we are using are BW25113, DH5a and BL21(DE3). Those E. colis are used for produce proteins(nacrein) and they use different promoters. Refer to former research they studied widely about safety and by the result founded to be very safe. They also weakened for laboratory use. Therefore would not pose a threat to environment. Thus, this E. coli strain is less likely to harm researchers. <br /><br /> | ||
| Line 24: | Line 24: | ||
http://bric.postech.ac.kr/myboard/read.php?Board=safety&id=1481(national guideline, Korean) | http://bric.postech.ac.kr/myboard/read.php?Board=safety&id=1481(national guideline, Korean) | ||
| - | <li> | + | <li>Vectors</li> |
Vectors We used is pATLIC and pBL plasmid. They used to produce protein which we want to made by using other promoters. pATLIC and pBL vector have same role but protein which made by pBL system is soluble but protein made by pATLIC is insoluble. <br /><br /> | Vectors We used is pATLIC and pBL plasmid. They used to produce protein which we want to made by using other promoters. pATLIC and pBL vector have same role but protein which made by pBL system is soluble but protein made by pATLIC is insoluble. <br /><br /> | ||
This plasmid have resist genes against antibiotics. So it can be danger to nature and human. however to transcript DNA and make protein, they need some key materials. So in nature they can be activated. Also we follow safety protocols about definite by our university. Its include biohazard treatment so we can isolate our vector from nature and human. | This plasmid have resist genes against antibiotics. So it can be danger to nature and human. however to transcript DNA and make protein, they need some key materials. So in nature they can be activated. Also we follow safety protocols about definite by our university. Its include biohazard treatment so we can isolate our vector from nature and human. | ||
</ol> | </ol> | ||
| - | <li> | + | <li>Risks to the safety and health of the general public</li> |
There are no significant risks to the safety and health of the general public. First, we do not use pathogenic bacteria. Second, gene products do not induce immune response. Third, our lab follows rules of bio-safety level limiting reagents and material we use. Here are reasons why our project is less likely to cause any health risks. | There are no significant risks to the safety and health of the general public. First, we do not use pathogenic bacteria. Second, gene products do not induce immune response. Third, our lab follows rules of bio-safety level limiting reagents and material we use. Here are reasons why our project is less likely to cause any health risks. | ||
</div> | </div> | ||
Revision as of 22:29, 27 September 2013
Would any of your project ideas raise safety issues in terms of:
- Issues to the researcher safety
- E.coli(BW25113, DH5a, BL21(DE3) Moreover, E. coli strain we are using are BW25113, DH5a and BL21(DE3). Those E. colis are used for produce proteins(nacrein) and they use different promoters. Refer to former research they studied widely about safety and by the result founded to be very safe. They also weakened for laboratory use. Therefore would not pose a threat to environment. Thus, this E. coli strain is less likely to harm researchers.
- Vectors Vectors We used is pATLIC and pBL plasmid. They used to produce protein which we want to made by using other promoters. pATLIC and pBL vector have same role but protein which made by pBL system is soluble but protein made by pATLIC is insoluble.
- Risks to the safety and health of the general public There are no significant risks to the safety and health of the general public. First, we do not use pathogenic bacteria. Second, gene products do not induce immune response. Third, our lab follows rules of bio-safety level limiting reagents and material we use. Here are reasons why our project is less likely to cause any health risks.
In conclusion, we find our project harmless. Although no significant safety issues were raised during the experiment, it is advised that experimenter should follow safety protocols listed on this website. This includes wearing gloves and goggles as bacteria may cause irritation and skin rash. Please refer to lab safety protocol: http://kusafe.korea.ac.kr/main.asp (university guideline, Korean) http://bric.postech.ac.kr/myboard/read.php?Board=safety&id=1481(national guideline, Korean)
This plasmid have resist genes against antibiotics. So it can be danger to nature and human. however to transcript DNA and make protein, they need some key materials. So in nature they can be activated. Also we follow safety protocols about definite by our university. Its include biohazard treatment so we can isolate our vector from nature and human.
