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ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1> Name of participants: Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis: 13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Gel electrophoresis: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m PCR amplification of construct: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature ) Lb13. Lc. lactis MG1363 pJP059 Competent cells preparation: Lb11. plantarum 36E no plasmid Lb10. plantarum 256 noplasmid Lb12. reuteri DSM 20016 no plasmid Plasmid preparation: Lb6.1 reuteri DSM 20016 pLUL634TsA8 Lb6.2 reuteri DSM 20016 pLUL634TsA8 Lb6.3 reuteri DSM 20016 pLUL634TsA8 Lb6.4 reuteri DSM 20016 pLUL634TsA8 * Lb7.2 reuteri DSM 20016 pGFP Lb7.3 reuteri DSM 20016 pGFP Lb7.4 reuteri DSM 20016 pGFP * Lb14.1 reuteri DSM 20016 pLUL631 Lb14.2 reuteri DSM 20016 pLUL631 Lb14.3 reuteri DSM 20016 pLUL631 Lb14.4 reuteri DSM 20016 pLUL631 * * 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml. O/N: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid Streaking of strains: Lb13. Lc. lactis MG1363 pJP059 Lb15. Lc. lactis MG1363 pGus Lb16. Lc. lactis MG1363 no plasmid PCR amplification: Lb13. Lc. lactis MG1363 pJP059* *Another attempt at colony PCR with different concentrations of lysozyme and buffer. <h2>Results</h2> Gel electrophoresis: 15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25) Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture. Competant cells: Wrong volume was added. → Try again tomorrow with the right volume. Plasmid preparation: * Lb6.1 reuteri DSM 20016 pLUL634 TsA8: 203.7 ng/µl Lb6.2 reuteri DSM 20016 pLUL634 TsA8: 203.2 ng/µl Lb6.3 reuteri DSM 20016 pLUL634 TsA8: 154.8 ng/µl Lb6.4 reuteri DSM 20016 pLUL634 TsA8: 39.5 ng/µl Lb7.2 reuteri DSM 20016 pGFP: 100.5 ng/µl Lb7.3 reuteri DSM 20016 pGFP: 141.2 ng/µl Lb7.4 reuteri DSM 20016 pGFP: 8.7 ng/µl Lb14.1 reuteri DSM 20016 pLUL631: 162.7 ng/µl Lb14.2 reuteri DSM 20016 pLUL631: 85.2 ng/µl Lb14.3 reuteri DSM 20016 pLUL631: 49.8 ng/µl Lb14.4 reuteri DSM 20016 pLUL631: 7.7 ng/µl *The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones. <h2>Other experiments</h2> Preparation of MRS broth Making plates for Lc. lactis cultivation (from M17-broth) Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</div>'

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{

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ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1> Name of participants: Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 E. coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Plasmid preparation: Lb8. reuteri 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 Digestion of construct: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m O/N (from frozen stock): Lb2. 1063 pLUL631 Lb6. DSM 20016 pLUL63TsA8 Lb7. DSM 20016 pGFP Lb8. 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 PCR: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT* Lb13. Lc. lactis MG1363 pJP059* *Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: 1.1. pSB1C3-red 18.2. pSB1C3-CHS_m 40. pEL3A17-red <h2>Results</h2> Plasmid preparation: Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today. <h2>Other experiments</h2> Primer stock preparation Dilution of sequencing primers: 3 tubes of 5 µM VF2-primer, 200 uL 3 tubes 5 µM VR-primer, 200 µL Re-measuring concentration of earlier plasmid preps: * 1.4 CF48-pLR581: 127.8 ng/µl 1.5 CF48-pLR581: 215.9 ng/µl 3.2 DSM 20016-pVs2: 201.2 ng/µl 3.3 DSM 20016-pVs2: 139.1 ng/µl 3.10 DSM 20016-pVs2: 183.5 ng/µl *We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind. Preparing M17-broth, 950 ml. (“) SOB (“) CCMB80 (20% glycerol, pH 6,8) Alliquoting dNTP, buffer, enzymes</div>'

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   {
 	ds = '<div id="dairy-text"><h1>Tuesday 2013-06-25</h1><br><br><b>Name of participants:</b> Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Theodor L, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis:</b><br>13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Gel electrophoresis:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>PCR amplification of construct:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT (second try, using smaller concentration of template and higher annealing temperature )<br>Lb13. Lc. lactis MG1363 pJP059<br><br><b>Competent cells preparation: </b><br>Lb11. plantarum 36E no plasmid<br>Lb10. plantarum 256 noplasmid<br>Lb12. reuteri DSM 20016 no plasmid<br><br><b>Plasmid preparation: </b><br>Lb6.1 reuteri DSM 20016 pLUL634TsA8<br>Lb6.2 reuteri DSM 20016 pLUL634TsA8<br>Lb6.3 reuteri DSM 20016 pLUL634TsA8<br>Lb6.4 reuteri DSM 20016 pLUL634TsA8 *<br>Lb7.2 reuteri DSM 20016 pGFP<br>Lb7.3 reuteri DSM 20016 pGFP<br>Lb7.4 reuteri DSM 20016 pGFP *<br>Lb14.1 reuteri DSM 20016 pLUL631<br>Lb14.2 reuteri DSM 20016 pLUL631<br>Lb14.3 reuteri DSM 20016 pLUL631<br>Lb14.4 reuteri DSM 20016 pLUL631 *<br>* 6.4, 7.4 and 14.4 were treated in a different way than the regular plasmid prep protocol for L. reuteri. Instead of treating them with LYSIS-buffer and lysozyme they were treated with resuspension solution and lysis solution from the plasmid prep kit and allowed to lyse for ~30 minutes. The rest were treated as usual, but with 1 ml of 10 mg/ml lysozyme instead of 2 ml.<br><br><b>O/N:</b> <br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>Streaking of strains:</b><br>Lb13. Lc. lactis MG1363 pJP059<br>Lb15. Lc. lactis MG1363 pGus<br>Lb16. Lc. lactis MG1363 no plasmid<br><br><b>PCR amplification: </b><br>Lb13. Lc. lactis MG1363 pJP059*<br>*Another attempt at colony PCR with different concentrations of lysozyme and buffer.<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>15. failed. Possibly nonspecific primer binding, too much template DNA. Image 1 (2013.06.25)<br>Lb13. Lc. lactis MG1363 pJP059: Only short bands, probably primer dimers because lysing failed. → Try again with a plasmid prep instead of culture.<br><br><b>Competant cells:</b><br>Wrong volume was added. → Try again tomorrow with the right volume.<br><br><b>Plasmid preparation: *</b><br>Lb6.1 reuteri DSM 20016 pLUL634 TsA8:	203.7 ng/µl<br>Lb6.2 reuteri DSM 20016 pLUL634 TsA8:	203.2 ng/µl<br>Lb6.3 reuteri DSM 20016 pLUL634 TsA8:	154.8 ng/µl<br>Lb6.4 reuteri DSM 20016 pLUL634 TsA8:	39.5 ng/µl<br>Lb7.2 reuteri DSM 20016 pGFP:		100.5 ng/µl<br>Lb7.3 reuteri DSM 20016 pGFP:		141.2 ng/µl<br>Lb7.4 reuteri DSM 20016 pGFP:		8.7 ng/µl<br>Lb14.1 reuteri DSM 20016 pLUL631:	162.7 ng/µl<br>Lb14.2 reuteri DSM 20016 pLUL631:	85.2 ng/µl<br>Lb14.3 reuteri DSM 20016 pLUL631:	49.8 ng/µl<br>Lb14.4 reuteri DSM 20016 pLUL631:	7.7 ng/µl<br>*The alternative way of lysing the cells proved to be quite ineffective so we will continue to use our standard protocol. The earlier plasmid preparations of the above “constructs”, that had far poorer concentrations, were replaced with these better ones.<br><br><h2>Other experiments</h2><br><b>Preparation of MRS broth </b><br><br><b>Making plates for Lc. lactis cultivation (from M17-broth)</b><br><br><b>Preparation of homemade yogurt from dried bacteria and from commercial yogurt with active bacteria culture</b></div>'
+ }
+ else if(id == 'd2013624')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1><br><b>Name of participants: </b>Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D.<br><br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m	<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>Lb8. reuteri 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Digestion of construct:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>O/N (from frozen stock): </b><br>Lb2. 1063 pLUL631<br>Lb6. DSM 20016 pLUL63TsA8<br>Lb7. DSM 20016 pGFP<br>Lb8. 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>PCR:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT*<br>Lb13. Lc. lactis MG1363 pJP059*<br>*Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. <br><br><b>Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: </b><br>1.1. pSB1C3-red<br>18.2. pSB1C3-CHS_m<br>40. pEL3A17-red<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl<br>Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl<br>Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today.<br><br><h2>Other experiments</h2><br><b>Primer stock preparation</b><br><br><b>Dilution of sequencing primers: </b><br>3 tubes of 5 µM VF2-primer, 200 uL<br>3 tubes 5 µM VR-primer, 200 µL<br><br><b>Re-measuring concentration of earlier plasmid preps: *</b><br>1.4 CF48-pLR581: 127.8 ng/µl<br>1.5 CF48-pLR581: 215.9 ng/µl<br>3.2 DSM 20016-pVs2: 201.2 ng/µl<br>3.3 DSM 20016-pVs2: 139.1 ng/µl<br>3.10 DSM 20016-pVs2: 183.5 ng/µl<br>*We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind.<br><br><b>Preparing M17-broth, 950 ml.</b><br><b>(“) SOB</b><br><b>(“) CCMB80 (20% glycerol, pH 6,8)</b><br><b>Alliquoting dNTP, buffer, enzymes</b></div>'
   }
   else if(id == 'd201373')

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