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Team:UANL Mty-Mexico/Safety/stability test
From 2013.igem.org
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<p>Plasmid stability test in E. coli:</p> | <p>Plasmid stability test in E. coli:</p> | ||
<p>A. Inoculate a flask with 10 ml of LB medium without antibiotic.</p> | <p>A. Inoculate a flask with 10 ml of LB medium without antibiotic.</p> | ||
Revision as of 23:12, 27 September 2013
Stability Test
Protocol
Plasmid stability test in E. coli:
A. Inoculate a flask with 10 ml of LB medium without antibiotic.
B. Grow overnight until saturation is reached.
C. Make serial dilutions (1:6, 1:7, 1:8).
D. Inoculate in LB medium without antibiotic and incubate for 80 Generations.
E. Inoculate each generation in petri dishes with LB medium with and without antibiotic, incubate overnight.
F. Count colonies and calculate the percentage of plasmid retention.
