"
Team:Northwestern/makem9
From 2013.igem.org
(Difference between revisions)
2013.igem.nu (Talk | contribs)
(Created page with "{{:Team:Northwestern/Templates/Skinning}} <html> <style> p { color:black; font-family: helvetica; } .container { display: inline-block; background-color: #C3...")
Newer edit →
(Created page with "{{:Team:Northwestern/Templates/Skinning}} <html> <style> p { color:black; font-family: helvetica; } .container { display: inline-block; background-color: #C3...")
Newer edit →
Revision as of 23:24, 27 September 2013
Make M9
M9 minimal media - Store at 4C:
- 1x M9 salts (From 5X autoclaved stock)
- 2mM MgSO4 (From 1M autoclaved stock)
- 0.1mM CaCl2 (From 1M autoclaved stock)
- 0.4% carbon source (From 40% glucose filtered stock)
- 0.25ug/ml Biotin (From filtered 1000X, 0.25mg/ml stock) - EcNR2-specific
- 0.00005% Vitamin B1 (thiamine) (From 10,000X, 0.5% filtered stock) - MG1655-specific (see Jensen, J. Bacteriol., 1993)
Bring to volume with sterile water
5X M9 salts:- 64g Na2HPO4*7H2O
- 15g KH2PO4
- 2.5g NaCl
- 5g NH4Cl
